LOCUS       sequence-319808-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-125a~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir125~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..62
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     13..33
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
       61 ctggtttagt gaaccgtcag atccgctagg gatcctctag tcagctgacg cgtgctagcg
      121 cggccgccgt ttttggtctt tctgtctctg gctctcagaa tgtctctgtg cctatctcca
      181 tctctgaccc ccaccccagg gtctaccggg ccaccgcaca ccatgttgcc agtctctagg
      241 tccctgagac cctttaacct gtgaggacat ccagggtcac aggtgaggtt cttgggagcc
      301 tggcgtctgg cccaaccaca cacctgggga attgctggcc tgacttctga cccctgactc
      361 ctcataccct tcctccagag catgacattt gaccaccaac tgaaacctga caagaagctc
      421 tctgctcagc ctgtcacaac ctactgactg ccagggcact tgggaatggc aaggaaaccg
      481 ttaccattac tgagtttagt aatggtaatg gttctcttgc tatacccaga aaacgtgcca
      541 ggaagagaac tcaggaccct gaagcagact actggaaggg agactccaaa gcttgtcgac
      601 gatatctcca gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      661 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      721 acttgtttat t
//
