LOCUS       sequence-319809-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-181b-1 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir181b-1-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    384..465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(421..440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(476..493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg acgcgtgcta
       61 gcgcggccgc aaaccaaagg tggacacacc aggactttct cttccctgtg cagagattat
      121 tttttaaaag gtcacaatca acattcattg ctgtcggtgg gttgaactgt gtggacaagc
      181 tcactgaaca atgaatgcaa ctgtggcccc gctttttgct gtcacaatca acagatattc
      241 catctttgaa agaagcttgt cgacgatatc tccagaggat cataatcagc cataccacat
      301 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      361 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      421 gcaatagcat cacaaatttc acaaataaag catttttttc actgccccga gcttcctcgc
      481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca ga
//
