LOCUS       sequence-319810-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-181b-1~125a and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir181b-1~125-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..61
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     12..32
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(761..780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tggtttagtg aaccgtcaga tccgctaggg atcctctagt cagctgacgc gtgctagcgc
      121 ggccgcaaac caaaggtgga cacaccagga ctttctcttc cctgtgcaga gattattttt
      181 taaaaggtca caatcaacat tcattgctgt cggtgggttg aactgtgtgg acaagctcac
      241 tgaacaatga atgcaactgt ggccccgctt tttgctgtca caatcaacag atattccatc
      301 tttgaaagcg tttttggtct ttctgtctct ggctctcaga atgtctctgt gcctatctcc
      361 atctctgacc cccaccccag ggtctaccgg gccaccgcac accatgttgc cagtctctag
      421 gtccctgaga ccctttaacc tgtgaggaca tccagggtca caggtgaggt tcttgggagc
      481 ctggcgtctg gcccaaccac acacctgggg aattgctggc ctgacttctg acccctgact
      541 cctcataccc ttcctccaga gcatgacatt tgaccaccaa ctgaaacctg acaagcttgt
      601 cgacgatatc tccagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
      661 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      721 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      781 acaaataaag c
//
