LOCUS       sequence-319812-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-421 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir421-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    470..551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(507..526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(562..579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
       61 gggatcctct agtcagctga cgcgtgctag cgcggccgcc tatggctctc gtctaccaga
      121 ctttaaattc cttaagggca aggacagtgc cttactcatc tttgtattca cagtgcctaa
      181 tccggtgcac attgtaggcc tcattaaatg tttgttgaat gaaaaaatga atcatcaaca
      241 gacattaatt gggcgcctgc tctgtgatct ccatgggctc agcttgtccc cgccagttgc
      301 caacaacgtc caagctctct tcagaatgct tactcctgaa gcttgtcgac gatatctcca
      361 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      421 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      541 tttttcactg ccccgagctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      721 gttgctggcg tttttccata ggc
//
