LOCUS       sequence-319813-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-374b~421 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir374b~421-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    642..723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(679..698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(734..751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggtaagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
       61 gatccgctag ggatcctcta gtcagctgac gcgtgctagc gcggccgcag cacgtgacaa
      121 aaacaacagc agaccctggt gcctgggagg acttcatgga tccagcagca acctggagtg
      181 gtgctcctct gaagaaatcc tactcggatg gatataatac aacctgctaa gtgtcctagc
      241 acttagcagg ttgtattatc attgtccgtg tctatggctc tcgtctacca gactttaaat
      301 tccttaaggg caaggacagt gccttactca tctttgtatt cacagtgcct aatccggtgc
      361 acattgtagg cctcattaaa tgtttgttga atgaaaaaat gaatcatcaa cagacattaa
      421 ttgggcgcct gctctgtgat ctccatgggc tcagcttgtc cccgccagtt gccaacaacg
      481 tccaagctct cttcagaatg cttactcctg aagcttgtcg acgatatctc cagaggatca
      541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      721 tgccccgagc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      841 agaacatgtg agcaaaaggc ca
//
