LOCUS       sequence-319815-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144(mutant2)~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144m2~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    568..649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(605..624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(660..677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagggatc ctctagtcag ctgacgcgtg
       61 ctagcgcggc cgcgttctgc ccccagcccc tcacagtgct tttcaagcca tgcttcctgt
      121 gcccccagtg actacctggc tgggatatca tcatatactg taagtttgcg atgagacact
      181 acagtataga tgatgtacta gtccggtaag tcccagctct ggagcctgac aaggaggaca
      241 ggagagatgc tgcaagccca agaagctctc tgctcagcct gtcacaacct actgactgcc
      301 agggcacttg ggaatggcaa ggaaaccgtt accattactg agtttagtaa tggtaatggt
      361 tctcttgcta tacccagaaa acgtgccagg aagagaactc aggaccctga agcagactac
      421 tggaagggag actccaaagc ttgtcgacga tatctccaga ggatcataat cagccatacc
      481 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      541 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      601 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgcc ccgagcttcc
      661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaag
//
