LOCUS       sequence-319816-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144(mutant3)~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144m3~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    565..646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(602..621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(657..674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg acgcgtgcta
       61 gcgcggccgc gttctgcccc cagcccctca cagtgctttt caagccatgc ttcctgtgcc
      121 agctgtgggg ccctggctgg gatatcatca tatactgtaa gtttgcgatg agacactaca
      181 gtatagatga tgtactagtc cgggcacccc cagctctgga gcctgacaag gaggacagga
      241 gagatgctgc aagcccaaga agctctctgc tcagcctgtc acaacctact gactgccagg
      301 gcacttggga atggcaagga aaccgttacc attactgagt ttagtaatgg taatggttct
      361 cttgctatac ccagaaaacg tgccaggaag agaactcagg accctgaagc agactactgg
      421 aagggagact ccaaagcttg tcgacgatat ctccagagga tcataatcag ccataccaca
      481 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      541 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgccccg agcttcctcg
      661 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactca
//
