LOCUS       sequence-319817-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144(mutant4)~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144m4~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    620..701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(657..676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(712..729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ggtttagtga accgtcagat ccgctaggga tcctctagtc agctgacgcg tgctagcgcg
      121 gccgcgttct gcccccagcc cctcacagtg cttttcaagc catgcttcct gtgcccccag
      181 tggggccctg gctgggatat catcatatac tgtaagtttg cgatgagaca ctacagtata
      241 gatgatgtac tagtccgggc accccctggg ctggagcctg acaaggagga caggagagat
      301 gctgcaagcc caagaagctc tctgctcagc ctgtcacaac ctactgactg ccagggcact
      361 tgggaatggc aaggaaaccg ttaccattac tgagtttagt aatggtaatg gttctcttgc
      421 tatacccaga aaacgtgcca ggaagagaac tcaggaccct gaagcagact actggaaggg
      481 agactccaaa gcttgtcgac gatatctcca gaggatcata atcagccata ccacatttgt
      541 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      601 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      661 tagcatcaca aatttcacaa ataaagcatt tttttcactg ccccgagctt cctcgctcac
      721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      781 aatacggtta tccacagaat caggggataa cgcanga
//
