LOCUS       sequence-319818-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144(mutant5)~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144m5~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    563..644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(600..619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(655..672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag ggatcctcta gtcagctgac gcgtgctagc
       61 gcggccgcgt tctgccccca gcccctcaca gtgcttttca agccatgctt cctgtgccag
      121 ctgtggggcc ctggctggga tatcatcata tactgtaagt ttgcgatgag acactacagt
      181 atagatgatg tactagtccg ggcaccccct gggctggagc ctgacaagga ggacaggaga
      241 gatgctgcaa gcccaagaag ctctctgctc agcctgtcac aacctactga ctgccagggc
      301 acttgggaat ggcaaggaaa ccgttaccat tactgagttt agtaatggta atggttctct
      361 tgctataccc agaaaacgtg ccaggaagag aactcaggac cctgaagcag actactggaa
      421 gggagactcc aaagcttgtc gacgatatct ccagaggatc ataatcagcc ataccacatt
      481 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      541 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      601 caatagcatc acaaatttca caaataaagc atttttttca ctgccccgag cttcctcgct
      661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      721 ggtaatacgg ttatccacag aatcag
//
