LOCUS       sequence-319820-        5642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the SIR2 + HerA defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG024 (SIR2 + HerA)
FEATURES             Location/Qualifiers
     source          1..5642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2120..2167
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2387..3046)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2961..2980
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3047..3149)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3541..3558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3675..4220)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3755..3774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaatatggct ttcaatcaag taactgtagt tgggggagga agcaaggcat attttaatag
       61 tttcgtagaa catctaccat accctgtgct ctttccacga gataatattg ttgatgagtt
      121 ggttgaagca attgctaatc tttctaaagg agaaggtaat gtcccttttt aaacttactg
      181 aaatctcggc tattggatac gttgtaggat tagaagggga aagaattagg ataaacctgc
      241 atgaggggtt gcaaggcaga ttagcatcgc atagaaaggg ggtgagctca gtaacgcaac
      301 caggagatct tattgggttc gatgcaggta atatattagt tgtcgcaaga gtgacagata
      361 tggcatttgt tgaagcggat aaagcgcata aggcaaatgt aggcacatct gatttagctg
      421 atatacctct aagacaaatt atcgcctatg caattggctt tgtgaaaagg gagttaaatg
      481 gttatgtttt tatatcagaa gattggcgct tacctgcatt gggttcttct gctgttcctt
      541 tgacttcaga ttttttgaac atcatttata gtattgataa agaagaactc ccaaaagcgg
      601 ttgaattagg tgtggattct agaactaaaa ccgttaagat atttgcaagt gttgataaat
      661 tattgtcgcg acacttagcc gttcttggta gtacaggata tggtaaatca aatttcaatg
      721 ctttgttaac gaggaaggtt tctgaaaaat accctaactc aagaatagtt atttttgaca
      781 taaatggtga atacgcgcaa gcttttacag gtattccaaa tgtaaagcac actattctag
      841 gggaatcccc aaatgttgat agtttggaaa aaaagcagca aaagggtgag ctatatagtg
      901 aagagtatta ttgttataaa aagataccat atcaggcatt aggttttgct gggttaatta
      961 aattattaag accaagtgat aaaacacaat tgcccgcatt aagaaatgca ttaagtgcaa
     1021 ttaatcggac tcattttaaa agccgtaata tttacttgga aaaagatgat ggtgaaactt
     1081 ttcttttgta tgatgattgt cgtgacacaa atcaaagtaa attggctgag tggttggatt
     1141 tattaaggcg tagacgtctt aaaagaacga atgtatggcc accgtttaaa agtttagcga
     1201 ctttggttgc tgaatttgga tgtgtagctg ctgaccgttc taatggaagt aaacgtgacg
     1261 cgtttggttt tagtaacgtg ttgccattgg taaaaatcat acaacaactt gcagaggata
     1321 taagatttaa atctattgtt aatttaaatg gagggggtga gctagcagat ggtggaacgc
     1381 attgggataa agctatgagt gatgaagttg attacttctt tggtaaggaa aaaggacaag
     1441 aaaatgattg gaatgttcat atagttaata tgaaaaattt ggcacaagat catgctccaa
     1501 tgttacttag tgcattgttg gagatgtttg ctgagatact atttagacgt gggcaggaac
     1561 gttcgtatcc tacggtactt ttgttggaag aagcgcatca ttacctgcgt gacccttatg
     1621 ctgaaattga ctcacagatt aaagcatatg aacgacttgc taaagaaggt aggaaattca
     1681 aatgctcttt aattgtcagt actcagcgac cctcagagct ttctcctact gttttggcaa
     1741 tgtgttcaaa ctggttttcg ttacgtttga ctaatgaaag agatttacag gctctcagat
     1801 atgcaatgga aagcggtaat gaacaaatct taaaacaaat atcaggttta ccaagaggtg
     1861 atgctgttgc atttggttct gcatttaatt tgcctgtaag aatttcaatt aatcaagcaa
     1921 ggccagggcc aaaatcttca gatgctgttt tttctgaaga atgggctaat tgtacagaat
     1981 tacgttgtta attacctgat gtacatggct agtgcaagtt ggtagcgcat gtctatatgc
     2041 atttatttgc atgtgtttta ttgagtgagc gcacaagctt gatgacccga caggtatgta
     2101 tttagactga acgcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     2161 ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     2221 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     2281 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     2341 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     2401 gccactcatc gcaatactgt tgtaattcat ttaacattct gccgacatgg aagccatcac
     2461 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2521 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2581 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2641 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2701 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2761 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcatcg
     2821 ccatacggaa ctctggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2881 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2941 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     3001 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     3061 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     3121 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     3181 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     3241 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     3301 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     3361 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     3421 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     3481 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     3541 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     3601 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     3661 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     3721 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     3781 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     3841 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     3901 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     3961 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     4021 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     4081 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     4141 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     4201 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     4261 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     4321 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     4381 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     4441 gcgcatagct attgtgacta tgctaaccat atgaatctat tgtgtgatta tgagtaatga
     4501 ctttttctaa tatttgattt ttaatgtagt aacttagcta attttaaaat ttgtaaaagg
     4561 atgtttatgt cgatttatca aggtggtaac aagttaaatg aggatgattt tcgttctcac
     4621 gtttattcct tgtgtcaatt agataatgtt ggcgttctgt taggtgctgg tgcttctgtc
     4681 ggttgtggtg ggaaaacgat gaaagatgta tggaaatcgt ttaagcaaaa ctaccctgag
     4741 cttttgggag cacttattga taaatatctt ctggtttcgc aaattgattc tgataacaat
     4801 ttggtcaatg ttgaactttt gatagatgaa gcaactaaat ttctttctgt agctaaaact
     4861 agacgatgtg aagatgaaga ggaggaattc aggaaaatat taagttcatt atataaagag
     4921 gttacgaagg ctgcattatt aacaggagaa cagtttagag agaaaaatca gggtaaaaaa
     4981 gatgcgttta aatatcacaa agagttaatt tcaaaattaa tttcaaatag acagcccggt
     5041 cagtcggctc cggcaatttt tacaacaaat tatgatttgg ccttagagtg ggctgcagaa
     5101 gatttaggaa tacagttgtt taatggtttt tctgggctac atacacggca gttttatccc
     5161 cagaattttg atttggcttt cagaaatgta aatgcgaagg gcgaagcaag attcggacat
     5221 tatcatgcgt atctctataa attacatggc tcacttacgt ggtatcaaaa tgatagcttg
     5281 actgttaacg aagttagtgc atctcaagca tatgatgaat atattaatga cataatcaat
     5341 aaagatgact tttatcgcgg tcaacatttg atttatccag gggcgaataa atatagccat
     5401 acaatcggct tcgtttatgg agagatgttt agacgttttg gggagtttat ttcgaaacct
     5461 caaacagcgt tgttcataaa tgggtttggt ttcggtgatt atcatataaa tagaataata
     5521 ttaggcgcgt tactgaatcc atctttccat gttgttatat attatcctga attgaaagaa
     5581 gcaattacca aagtaagtaa gggtggtggt tcggaagctg agaaagctat tgttacttta
     5641 aa
//
