LOCUS       sequence-319821-        7442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG018 (AVAST type 2)
FEATURES             Location/Qualifiers
     source          1..7442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(249..908)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     823..842
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(909..1011)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1403..1420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1537..2082)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1617..1636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      join(7425..7442,1..29)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
       61 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
      121 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
      181 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
      241 taaaaaaatt acgccccgcc ctgccactca tcgcaatact gttgtaattc atttaacatt
      301 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
      361 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
      421 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
      481 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      541 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      601 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      661 accagctcac cgtctttcat cgccatacgg aactctggat gagcattcat caggcgggca
      721 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      781 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
      841 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
      901 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
      961 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     1021 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     1081 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
     1141 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
     1201 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
     1261 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
     1321 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
     1381 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     1441 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
     1501 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
     1561 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     1621 ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     1681 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1741 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     1801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     1861 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     1921 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     1981 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     2041 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
     2101 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
     2161 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
     2221 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     2281 gctaacttac attaattgcg ttgcgcaggg aytkccacca cnncccaccg accatctaag
     2341 actttatgcc actgtcccta ggactgctat gtactaggag cggatgttaa actcagactc
     2401 gtttcagcta cattgcgttt tgaataatat tccatcataa taactctttg aaaaatgtga
     2461 tcttttcatt tataacactg atgacttgct tatctcattg ggatatcgga ggagaatact
     2521 taactatgac aagcccgatt attatgacac tggctatatt atatagattg atattaaaat
     2581 gtaggattag gttcttgcca aggtgtcaag atttacagat aggtttaaaa ccatataaat
     2641 atgttttacg gtgagataca atacatattg taaggcataa acgcttggta aaattttaat
     2701 tattggaaga agctaatcat ggaacccata tcaattacag tggcaactta tgtagcaact
     2761 aaacttattg atcaattcat ctctcaagaa ggatatggtt gtattaagaa agcattattc
     2821 ccccaaaaaa gatatgtgga tagattatat caactaattg aagagacggc aattgagttt
     2881 gaagaaacat atccagtaga aagtggagca ataccatttt atcattccga accattgttt
     2941 gagatgttga atgagcacat cttttttaaa gagttccctg acaaagagat attattagac
     3001 aagttcaaag aatatccaag tatcactccc ccaactcaac aacaactcag ccttttttat
     3061 gagatgttat cattaaaaat caataattgt tcgaagttaa aaaagctaca tatcgaagaa
     3121 acgtataaag aaaaaatatt cgatattaat gaagagctca ttcaagtcaa acttatttta
     3181 cggtctatag atgagaaact aacttttcac ttaagtgatg attggttaaa tgaaaaaaat
     3241 agtcaagcaa tagctgactt gggaggtcga tacacacccg aactcaacgt aaagctagaa
     3301 atagcagaga tatttgatgg cctcggtaga actaatgatt tttctaaaat attttattcg
     3361 catatagata gctttctggt cgctggaaag aaattacata gttgcgatgt aatttcctca
     3421 gaattatttg aaataaacca gtccttaaaa gaaatttctg atatatatca ggagattaat
     3481 ttttctaaat tagatgaaat ccctataaat aaatttaata actatgtttc tagctgccag
     3541 acagctattg gcggagcggt atcaatattg tgggaactcc gagaaaagtc agagcaagta
     3601 ggtgaaacca agcattacag tgataagtat tcatctactc tgcgaatgct tcgggaattt
     3661 gactatgcgt gcaatgaatt acgtatattc attaattcaa caacagtgaa gttggctaac
     3721 aacccattct tacttctcga aggaaaagca ggaattggta agtctcattt actggctgat
     3781 gtgattaaaa atcgaattgc ttctgggtat ccttcactac tcatactagg gcaacaactt
     3841 acttcagatg aatctccatg gtcacaaatc ttcaagagat tacagcttaa aatcacttct
     3901 cgtgaattcc tagaaaaact gaatttatat ggcaaaaaaa caggaaaaag agtcttagtt
     3961 tttattgatg ctattaatga aggtaatgga aataaattct ggaatgacaa tattaacagt
     4021 tttgtcgatg aaatcagatg ctttgaatgg cttggtctga taatgtcagt cagaacaaca
     4081 tatagaaatg taacaatttc acatgagaat gttgtgcgaa ataattttga aattcatgaa
     4141 catattggat tccagaacgt tgagttggaa gcggttagtc tattttatga ttattacaat
     4201 attgagaggc cttcatctcc taaccttaat ccagagttta aaaatcctct atttcttaag
     4261 ttattgtgtg aaggcattaa gaaaaatggt ttaaccaaag tgcctgttgg atttaatggg
     4321 atttcaaata tttttaactt tttagttgaa ggggtaaata aatcattagc atcgccaaaa
     4381 aaatatgcat tcgatcccag ttttcctctt gttaaagatg ctctcaatga aatcataaaa
     4441 ttcaaattag agattggtcg taatagtatt tcacttaaag atgctcactc agtggttcaa
     4501 tctgtagtta atgattatgt tgctgataaa accttcctca gcgccttgat tgacgaagga
     4561 ttattgacta aaggcatagt gagaaatgat gataattcta ctgaggaagt agtttatgtg
     4621 gcttttgaaa ggtttgatga tcatttaact gttaattttt tattaaatga tgttgaaaat
     4681 atcgaaagtg aatttaagcc tgatggtcgt ctgaaaaaat attttcatga tgaatgtgat
     4741 ttttatataa aatcgggaat agtagaggcg ttgtctattc aattgccaga aaggtatgaa
     4801 aaagagcttt atgaatttct gccggagttc agcaataatc ttaaattact agaagccttt
     4861 attgatagct tgatatggcg cgatattaag gctattgatt tcgaaaaaat tagacctttc
     4921 atcaatgaac atgtttttaa atttaaagat agttttgatc atttcctcga ggcagtgatc
     4981 tctatttcag gtttagttgg ccatcccttt aatgctaatt tcttgcatga ttggctaaaa
     5041 gattattctt tggcaaatcg agattcgttt tggactacag aacttaaata taaatatagt
     5101 gaagactcag catttaggca tctaatcgat tgggcatggg ccagaacaga taaaagcttt
     5161 gtttcggatg agtcaatcga gctagttgca actagtttat gctggttttt aacttctagt
     5221 aaccgagaac ttcgagattg ctcaactaag gctttagtga gtttactcga gccaagaatt
     5281 cctgtattga gaaaaataat tgataagttt tatggtgtaa atgatcctta cgtttgggaa
     5341 agaatatttg cagttgcatt aggctgtaca ttgcgaactg ataatattaa agaactaaaa
     5401 tatttagccg aaactgttta ccaaaaggta ttttgttcta agtatgtgta tccaaatata
     5461 ttacttagag attatgctag agagattatt gaatttgcta atcatcttgg attggaactt
     5521 gaaagcattg aattatccaa gactagacca ccctacaaca gcatttggcc tgacaagatt
     5581 ccttcaaaag aggaactaga gtccctttat gataaagaac cttatcggga actctggagc
     5641 tctattatgg aagatggtga cttttcacga tatactattg gaacaaatta taatcattct
     5701 gattggtctg gttgcaagtt taatgaaacc cctgttgacc gtaagcaagt ttttaaaact
     5761 ttcaaatgta aactaactga tcaacaaaaa gacttgtatg atgccacaga tcctttcatt
     5821 tatgatgata aatgcgaagg aattaaattt ggtcgtgtgg tcggtagaaa agcacaggaa
     5881 gaaataaagg cgagcaagaa attatttaag aattcattgt catacgatct gttaagtgag
     5941 tttgaaaatg aaatagagcc atacctggat cataataata atctgctgga aactgataaa
     6001 cactttgatc ttcgactagc tcaacaattt atattcaatc gtgttataga gcttggttgg
     6061 gatccggaga agcatggtaa ttttgaccaa caaataggaa ctggacgtgg acgtagagag
     6121 gcattccaag aacggattgg taaaaaatac caatggattg cttattatga atacatggca
     6181 aggctagccg ataattttac tcgttttgaa ggttatggtg acgaacgaaa ggaaaatcca
     6241 taccaagggc catgggagcc ttacgtaaga gatatagatc ccactatctt acttaaagaa
     6301 actggaacga aaaaaataag caataaagaa atgtggtggc ttaatgatga agtgtttgat
     6361 tggacttgct ctaatgaaga ctgggttaaa agttctacta ctataactaa ttcatatgct
     6421 tttattgaag ttaaagatga taatggtgat gaatggatag tattagaaag tcatccatca
     6481 tggaaagaac caaaaattat tggaaacgat gattgggggc acccacgaaa agaggtttgg
     6541 tatcagatca gaagttatat cgttaaagtt gaagaatttg aaaattttag atgttgggca
     6601 atagctcaag actttatggg caggtggatg ccggaatgta ctgatagata ccaattattt
     6661 aatagggagt actattggtc cgaagcwktt aagtctttta aatcagatta ttatggtgga
     6721 tctgactgga cttcggtaac agaccgggag tctggagcta agatagctga tgttagtgtc
     6781 acttcgatta attatttgtg ggaagaggag ttcgacaaat caaaaataga aactttgaat
     6841 tttttgaagc ctagtaactt aatctttgaa aagatgggat taaaaagtgg ggaagtagag
     6901 ggtagcttca atgatgaaaa tggaactatg gtttgctttg cagctgaagc tgtatatgct
     6961 tcaaagccgc atctacttgt taaaaaagaa ccatttttaa caatgttaag ggacaatggt
     7021 tttgaaatcg tttggacatt attaggtgaa aagggcgtta tagggggctc actcatatca
     7081 agtcatcatt atggtcgaca ggagtttagt ggagcatttt attatgaaga cagtcagcta
     7141 acaggaagtc ataaaactag ctttacgaga taaaaatgaa tctcagagct gaatatataa
     7201 gtagtattag aaaccgggtt atacttaaga aatcaatctt aagtgtggca gtcgaatggt
     7261 agctaatatg ctagcggcgc taatgcctgt ttgttgctca taacaggcat tcactttagt
     7321 tatggcagaa aagtatacat gctgggttgg gaaagtgtga aagaaaggaa gattgctgcg
     7381 ccgtttgtcg tcacgtttat cttcattggc tatgcacgca ataactagca taaccccttg
     7441 gg
//
