LOCUS       sequence-319822-        6806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6806)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6OGN
FEATURES             Location/Qualifiers
     source          1..6806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(995..1000,1001..1017,1018..1023))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1375..1466
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1391..1408
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1525..1542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1555..1575
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3786..3920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3823..3842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3877..3896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3949..4114
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4298..4752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4385..4404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4594..4615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4778..4882
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4883..4951,4952..5743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5101..5120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5914..6502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6403..6422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6656..6673
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      841 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      901 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      961 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     1021 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     1081 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     1141 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     1201 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     1261 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     1321 ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg
     1381 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1441 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1501 gcggatctcg gtccgaaaac catgcatcat catcatcatc acagcagcgg cagagaaaac
     1561 ttgtatttcc agggcaaggt cttctgtggc cgtgccaatc ctaccacggg atccctggag
     1621 tggctggagg aggatgaaca ctatgattac caccaggaga ttgccaggtc atcctatgcc
     1681 gacatgctac atgacaaaga cagaaatata aaatactacc agggtatccg ggcagctgtg
     1741 agcagggtga aagacagagg acagaaggcc ttggttcttg acattggcac tggcacaggc
     1801 ctcttgtcaa tgatggcagt tactgcaggg gctgacttct gctatgctat cgaggttttt
     1861 aagcctatgg ctgaggctgc tgtgaagatt gtggagagga atggcttcag tgataagatt
     1921 aaagtcatta acaagcactc cactgaggtg acagtcggac cagatggtga cttgccgtgt
     1981 cgtgctaaca ttctgatcac ggagctgttt gacacagagc tgattgggga gggagcgctg
     2041 ccctcttatg agcatgcaca caagcatctt gtccaggaag actgcgaggc agtgccacac
     2101 agggcaactg tctatgccca gctggtggag tcccgaagga tgtggtcctg gaacaagctg
     2161 tttcccgtcc gtgtccggac gagtctaggc gagcaggtca tcgtcccccc ctcagaattg
     2221 gagaggtgtc ctggtgcgcc ttcagtctgt gacattcagc tgaaccaggt gtcgcctgct
     2281 gacttcactg tcctcagtga tgtgctgcca atgttcagcg tggacttcag caagcaagtc
     2341 agcagctcgg cagcgtgcca tagcaggcag tttgtacctt tggcgtctgg ccaagcacag
     2401 gtggttctgt cctggtggga cattgaaatg gaccctgagg gcaagatcaa gtgcaccatg
     2461 gcaccctttt gggcacagac agatccgcag gagcttcagt ggcgggacca ctggatgcag
     2521 tgtgtgtact tcctgccgca ggaggagcct gttgtgcagg gctcaccccg gtgcctggta
     2581 gcccaccatg atgactactg tgtgtggtac agccttcaga gaaccagccc tgatgagaac
     2641 gacagcgcct accaagtgcg acctgtgtgt gactgtcagg ctcacttgct ctggaaccgg
     2701 cctcggtttg gagaaatcaa tgatcaggac agaactgatc actatgccca ggccctgagg
     2761 actgtgctgc tgccaggtag cgtctgcctt tgtgtgagtg atggcagtct cctctccatg
     2821 ctggcccatc acctcggagc ggagcaggtg tttacagttg agagttcagt agcttcctat
     2881 agactgatga aaaggatctt caaggttaac cacttggaag ataaaatcag tgtcatcaat
     2941 aaacggcctg agttgctgac agctgcagac ctggagggca agaaggtctc cctcctcctg
     3001 ggtgaaccct ttttcaccac cagcctgctg ccatggcaca acctgtactt ctggtatgtc
     3061 cgtacctctg tggaccagca cctagcacct ggagctgtgg tgatgcctca ggctgcctca
     3121 ctgcatgccg tgattgtgga gttcagggac ctgtggcgga tccggagtcc ttgcggtgac
     3181 tgcgaaggtt ttgatgtgca catcatggat gatatgatca agcactccct ggatttccga
     3241 gagagcagag aggcagagcc acacccactg tgggaatacc cctgcagaag cctctccaag
     3301 cctcaagaga tcctgacttt tgatttccag cagcccatcc cccaacagcc tatgcaatcc
     3361 aagggcacaa tggagctgac aagacccggg aagagccatg gggctgtcct gtggatggag
     3421 tatcagctca ctccagacag cacgatcagc actggcctca taaaccctgc agaagacaag
     3481 ggggactgct gctggaaccc ccactgcaag caagctgtgt acttcctcag caccacgctg
     3541 gatctcagag tgcctctgaa tggccctcgg tcagtcagct atgttgtgga gtttcacccc
     3601 ctcactggag acatcaccat ggagtttagg cttgcagaca ccttgagctg atgacgaagc
     3661 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     3721 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3781 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3901 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     3961 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     4021 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     4081 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     4141 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     4201 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     4261 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4561 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     4621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4741 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5041 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5101 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5161 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5221 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5281 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5341 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5401 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5461 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5521 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5581 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5641 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5701 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6601 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6661 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     6721 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     6781 ataaatggat gccctgcgta agcggg
//
