LOCUS       sequence-319823-        6890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6890)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6PDM
FEATURES             Location/Qualifiers
     source          1..6890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(995..1000,1001..1017,1018..1023))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1375..1466
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1391..1408
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1525..1542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1555..1575
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3870..4004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3907..3926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3961..3980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4033..4198
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4382..4836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4469..4488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4678..4699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4862..4966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4967..5035,5036..5827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5185..5204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5998..6586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6487..6506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6740..6757
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      841 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      901 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      961 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     1021 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     1081 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     1141 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     1201 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     1261 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     1321 ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg
     1381 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1441 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1501 gcggatctcg gtccgaaaac catgcatcat catcatcatc acagcagcgg cagagaaaac
     1561 ttgtatttcc agggcgcagt gaagctaaac cctgatttca gtgatgcaaa ggagaatttt
     1621 tatcgtgttg caaactggtt ggtggaacgc tggcacttta tcatgcttaa tgacaccaag
     1681 aggaatacaa tttataatgc agcaatccaa aaggcagttt gtttggggtc caaaagtgtt
     1741 ttggacattg gagcaggaac tggaatacta agcatgtttg ctaaaaaagc tggagcacat
     1801 tccgtgtatg cctgtgagtt atccaagacc atgtatgaac ttgcctgtga tgtcgtggca
     1861 gcaaacaaga tggaagcagg gatcaaactc ttacatacga agtcacttga catagagatt
     1921 ccaaaacata ttcccgaaag agtgtcccta gttgtaacag aaactgtcga tgcaggttta
     1981 tttggagaag gaattgtgga gagtttgatt catgcatggg agcatttact tttacagcca
     2041 aagaccaaag gtgaaagtgc taattgtgaa aagtatggga aagttatacc agcaagtgct
     2101 gttatatttg ggatggcagt agaatgtgca gagataagaa gacatcatag agtgggtatt
     2161 aaggacattg ctggtatcca tttgccaaca aatgtgaaat ttcagagtcc ggcttattct
     2221 tctgtagata ctgaagaaac aattgaacct tatacaactg aaaagatgag tcgagttcct
     2281 ggaggatatt tggctttgac agagtgcttt gaaattatga cagtagattt caacaacctt
     2341 caggaattaa aaagtcttgc aactaaaaag cctgataaga ttggtattcc tgttattaaa
     2401 gaaggcatac tagatgctat tatggtttgg tttgtgctcc agcttgatga tgaacatagt
     2461 ttatccacaa gtcctagtga ggaaacatgt tgggaacagg ctgtctaccc cgtacaggac
     2521 cttgcagact actggataaa gcctggagac catgtgatga tggaagtatc ttgtcaagac
     2581 tgttacttaa gaatccagag tattagtgtc ttgggtttgg aatgtgaaat ggatgttgca
     2641 aaaagtttta cccagaataa agacttgtta tcgttaggaa atgaggctga actttgtagt
     2701 gccctcgcta accttcagac cagtaaacca gatgctgtag agcagacatg tatattggaa
     2761 tctacagaaa ttgctttgct taacaacatc ccatatcatg aaggctttaa aatggcaatg
     2821 agcaaagttt tgtcttcact gactccagag aaactgtatc agaccatgga tactcactgt
     2881 cagaatgaga tgagctctgg aactggacag agtaatactg tacagaacat ccttgaacct
     2941 ttctacgtgt tagatgtgtc cgaaggcttc tctgttctgc ctgttattgc tggcacactt
     3001 gggcaggtta aaccatacag ttctgtggag aaagaccagc atcgtattgc tctggacctc
     3061 atatctgaag ccaatcactt tcctaaagaa acacttgagt tttggctgag acatgtggag
     3121 gatgaatctg ctatgttaca aaggccaaaa tcagacaagt tatggagcat aattatattg
     3181 gatgtcattg agccatctgg gctcattcag caggaaataa tggaaaaagc tgcaatatcc
     3241 aggtgtttac tacaatctgg aggcaagatc tttcctcagt atgtgctgat gtttgggttg
     3301 cttgtggaat cacagacact cctagaggag aatgctgttc aaggaacaga acgtactctt
     3361 ggattaaata tagcaccttt tattaaccag tttcaggtac ctatacgtgt atttttggac
     3421 ctatcctcat tgccctgtat acctttaagc aagccagtgg aactcttaag actagattta
     3481 atgactccgt atttgaacac ctctaacaga gaagtaaagg tatacgtttg taaatctgga
     3541 agactgactg ctattccatt ttggtatcat atgtaccttg atgaagagat taggttggat
     3601 acttcaagtg aagcctccca ctggaaacaa gctgcagttg ttttagataa tcccatccag
     3661 gttgaaatgg gagaggaact tgtactcagc attcagcatc acaaaagcaa tgtcagcatc
     3721 acagtaaagc aatgatgacg aagcttgtcg agaagtacta gaggatcata atcagccata
     3781 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3841 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgatatcgc
     4021 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
     4081 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     4141 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     4201 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     4261 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     4321 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     4381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     4681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4801 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6661 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6721 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6781 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     6841 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg
//
