LOCUS       sequence-319824-        5693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6PBG
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(67..600)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(770..775,776..792,793..798))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1150..1241
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1166..1183
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1300..1317
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1330..1350
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2448..2582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2485..2504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2539..2558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2611..2776
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2960..3414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3047..3066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3256..3277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3440..3544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3545..3613,3614..4405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3763..3782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4576..5164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5065..5084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5318..5335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5469..5693
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
       61 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
      121 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
      181 ataagcacca agcgcgttgg cctcatgctt gagcagattg atgagcgcgg tggcaatgcc
      241 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
      301 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
      361 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
      421 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
      481 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
      541 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
      601 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
      661 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
      721 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      781 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      841 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
      901 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
      961 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1021 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1081 tcgtttgttc gcccaggact ctagctatag ttctagtggt tggctacgta tactccggaa
     1141 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     1201 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     1261 gtcccaccat cgggcgcgga tctcggtccg aaaaccatgc atcatcatca tcatcacagc
     1321 agcggcagag aaaacttgta tttccagggc atgttaacca aattcgagac caagagcgcg
     1381 cgggtcaaag ggctcagctt tcaccccaaa agaccttgga tcctgactag tttacataat
     1441 ggggtcatcc agttatggga ctatcggatg tgcactctca ttgacaagtt tgatgaacat
     1501 gatggtccag tgcgaggcat tgacttccat aagcagcagc cactgttcgt ctctggagga
     1561 gatgactata agattaaggt ttggaattac aagcttcggc gctgtctttt cacattactt
     1621 gggcacttag attatattcg caccacgttt tttcatcatg aatatccctg gattctgagt
     1681 gcctccgatg atcagaccat ccgagtgtgg aattggcaat ctagaacctg tgtttgtgtg
     1741 ttaacagggc acaaccatta tgtgatgtgt gctcagttcc accccacaga agacttggta
     1801 gtatcagcca gcctggacca gactgtgcgc gtttgggata tttctggtct gaggaaaaaa
     1861 aacctgtccc ctggtgcggt ggaatcggat gtgagaggaa taactggggt tgatctattt
     1921 ggaactacag atgcagtggt gaagcatgta ctagagggtc acgatcgtgg agtaaactgg
     1981 gctgccttcc accccactat gccccttatt gtatctgggg cagatgatcg tcaagtgaag
     2041 atctggcgca tgaatgaatc aaaggcatgg gaggttgata cctgccgggg ccattacaac
     2101 aatgtatctt gtgccgtctt ccaccctcgc caagagttga tcctcagcaa ttctgaggac
     2161 aagagtattc gagtctggga tatgtctaag cggactgggg ttcagacttt ccgcagagac
     2221 catgatcgtt tctgggtcct agctgctcac cctaacctta acctctttgc agcaggccat
     2281 gatggtggta tgattgtgtt taagctggaa tgatgacgaa gcttgtcgag aagtactaga
     2341 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2401 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2581 tctgatcact gatatcgcct aggagatccg aaccagataa gtgaaatcta gttccaaact
     2641 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     2701 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     2761 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     2821 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     2881 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     2941 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3001 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3061 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3121 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3181 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3241 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3301 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3361 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3421 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3481 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3541 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3601 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3661 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3721 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3781 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3841 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3901 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3961 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4021 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4081 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4141 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4201 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4261 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4321 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4381 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4441 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4501 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4561 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4621 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4681 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4741 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4801 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4861 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4921 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4981 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5041 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5101 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5161 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5221 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5281 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5341 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5401 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
     5461 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     5521 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     5581 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     5641 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttc
//
