LOCUS       sequence-319825-        5756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6VYC
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1037..1057)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1070..1087)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(1146..1237)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(1204..1221)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(1589..1594,1595..1611,1612..1617)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1787..2320
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2387..2611
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2745..2762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2916..3504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2996..3015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3675..4466,4467..4535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4298..4317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4536..4640)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4666..5120)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4803..4824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5014..5033
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  5304..5469
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    5498..5632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5522..5541)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5576..5595
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcttcgtca tcaggcttta tgggccagga gggtggtcag cttgatcttg ccatccatgg
       61 aggcggtgag gcaggtccca cagtccatgg cagtgctcca gtccacggtg accacagggg
      121 ctcggtggcc acctaggctc aagcagctct ccagaacctt ctcatcgcca cccagcttgt
      181 agatgacgcc gcctgtggca gaacatgtca gcatgtaatt tccctccgag tcaaaagcga
      241 agagtcggcc cctggggact tgaacctgct tgtagccgct gtatccagac agcacaaagg
      301 ggcccgtggc atctgagggg aggctgtact cggatacctt gaggccactc ttgtggatgt
      361 tccactggat gaacttcccg tcctcgccga tgctgtacac ggtgttctca tcatagctga
      421 actccacaga gtagacctcc ccgtagtggg ccctccagct catcgcgcac tcatgctgct
      481 gcatgtcaaa cagccggatg acgccatcag ctgcccctgt gaccagcagg ttcccgttgt
      541 gattgaaggc tgtacagttg atagcaatgg gttctggatc cagggagaac tggagctgct
      601 gcttcatggt tttcgtgtcc cacagcagca gcctgccagg cacctgggaa gtgaggctcg
      661 gagctgctgc cgaacagacg aaagaggccc cgttggggct gcacgcaaga gacaggattc
      721 tgggcatgtt gtcgttgata ttgatttcac agagattctt cttggcttcc gtgtcataga
      781 gacgcactgt tcccacacca ctgcccagca agagcagtct gtcccgtttg gtggcccatt
      841 ccaaagacag cagcggtgat ttggaaatgg aggatgcttt ggtctgcatg atggggttga
      901 aggaccacac tttgatgacc ccatctacgt ctaagctggc gactctcctc ccagagcagt
      961 ccactctgca gtgcatgatg gatgagtggt gttccccgta ctcctcctgt cccagcacaa
     1021 taaagggctg ctcggggccc tggaaataca agttttctct gccgctgctg tgatgatgat
     1081 gatgatgcat ggttttcgga ccgagatccg cgcccgatgg tgggacggta tgaataatcc
     1141 ggaatattta taggtttttt tattacaaaa ctgttacgaa aacagtaaaa tacttattta
     1201 tttgcgagat ggttatcatt ttaattatct ccatgatcta ttaatattcc ggagtatacg
     1261 tagccaacca ctagaactat agctagagtc ctgggcgaac aaacgatgct cgccttccag
     1321 aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg
     1381 ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct tgccgtagaa
     1441 gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
     1501 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
     1561 gtggaaacgg atgaaggcac gaacccagtt gacataagcc tgttcggttc gtaaactgta
     1621 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1681 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
     1741 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1801 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg
     1861 catcattcgc acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga
     1921 tcttttcggt cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga
     1981 ttacctcggg aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga
     2041 agcggttgtt ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga
     2101 gatctatatc tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc
     2161 gctcatcaat ctgctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca
     2221 agcagattac ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga
     2281 agtgatgcac tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat
     2341 cggcttcccg gccgcggagt tgttcggtaa attgtcacaa cgccgcgaat atagtcttta
     2401 ccatgccctt ggccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
     2461 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
     2521 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
     2581 gttcagttta agactttatt gtccgcccac acccgcttac gcagggcatc catttattac
     2641 tcaaccgtaa ccgattttgc caggttacgc ggctggtcta tgcggtgtga aataccgcac
     2701 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     2761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     4681 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4741 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4801 ttccagtttg gaacaagagt ccactattaa gaacgtggac tccaacgtca aagggcgaaa
     4861 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4921 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4981 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5041 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5101 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg caaataagcg ttgatattca
     5161 gtcaattaca aacattaata acgaagagat gacagaaaaa ttttcattct gtgacagaga
     5221 aaaagtagcc gaagatgacg gtttgtcaca tggagttggc aggatgtttg attaaaaaca
     5281 taacaggaag aaaaatgccc cgctgtgggc ggacaaaata gttgggaact gggaggggtg
     5341 gaaatggagt ttttaaggat tatttaggga agagtgacaa aatagatggg aactgggtgt
     5401 agcgtcgtaa gctaatacga aaattaaaaa tgacaaaata gtttggaact agatttcact
     5461 tatctggttc ggatctccta ggcgatatca gtgatcagat ccagacatga taagatacat
     5521 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5581 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5641 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     5701 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagtacttc tcgaca
//
