LOCUS       sequence-319834-        6020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6020)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6WNX
FEATURES             Location/Qualifiers
     source          1..6020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1534..1668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1571..1590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1625..1644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1697..1862
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2046..2500
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2133..2152)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2342..2363
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2526..2630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2631..2699,2700..3491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2849..2868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3662..4250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4151..4170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4404..4421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4555..4779
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4846..5379)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5549..5554,5555..5571,5572..5577))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5929..6020
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5945..5962
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 attccggatt attcataccg tcccaccatc gggcgcggat ctcggtccga aaaccatgca
       61 tcatcatcat catcacagca gcggcagaga aaacttgtat ttccagggca tgttgcagcg
      121 ggactttatt accgctttac cagagcaagg cttagatcac atagcagaaa acattctttc
      181 gtacctggat gccaggtctc tgtgtgcagc agagctggta tgtaaagaat ggcagcgagt
      241 gatctcagaa ggaatgcttt ggaagaagct gattgaacga atggtacgca ctgatcccct
      301 atggaaagga ctttcagaaa gaagagggtg ggatcagtac ctgtttaaaa acagacccac
      361 agatggccct ccaaattcat tttataggtc attataccca aagattatcc aggatataga
      421 gactatagaa tctaactggc ggtgtggacg acacaacttg cagaggattc agtgccgctc
      481 tgaaaatagt aaaggtgtct actgtttaca gtacgatgat gaaaaaatta tcagtggcct
      541 acgagataat tctattaaga tatgggataa aaccagcctg gaatgtttga aagtgttaac
      601 aggacacaca ggctctgtcc tctgtctgca gtatgatgag cgtgtcattg taactggctc
      661 ttcagattct acggtgagag tgtgggatgt gaacacgggt gaagttctta acacattgat
      721 ccaccacaat gaggctgtat tgcacttacg cttcagcaat ggactgatgg tgacctgttc
      781 caaggaccgc tccattgctg tgtgggacat ggcttctgcg accgacatca ctttacgccg
      841 tgtcctggtt ggccaccggg ctgccgtcaa tgtagtagac tttgacgaca agtacatcgt
      901 gtctgcctct ggtgacagga ccatcaaagt ctggagcacg agcacctgtg aatttgttcg
      961 tactctcaat gggcacaagc ggggcattgc ctgtctccag tacagggatc gcctggttgt
     1021 tagtggatca tcagataata ccattaggct ctgggatatt gaatgtggtg cctgtttaag
     1081 agtcctagag ggacatgaag aattggtccg atgcatccgg tttgataaca agaggattgt
     1141 cagtggggcc tatgatggga aaattaaagt ttgggacttg caagctgctc ttgaccctcg
     1201 agccccagca agcacattgt gtttgcgcac attggtggaa cattctggac gtgtgtttcg
     1261 gctccagttt gatgagtttc agatcatcag cagctcccat gatgacacta ttttgatttg
     1321 ggatttctta aatgtgcctc ccagtgccca gaatgagacc cgttctccct ccagaacata
     1381 cacttacatc tctagatgat gacgaagctt gtcgagaagt actagaggat cataatcagc
     1441 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1501 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1561 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1621 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgata
     1681 tcgcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     1741 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     1801 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     1861 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     1921 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     1981 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
     2041 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     2101 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2161 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2221 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     2281 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
     2341 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2401 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2461 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     2521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     4441 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta
     4501 acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg
     4561 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     4621 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     4681 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     4741 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
     4801 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     4861 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     4921 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     4981 ctcatgcttg agcagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     5041 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     5101 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     5161 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     5221 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     5281 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     5341 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     5401 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
     5461 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     5521 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg
     5581 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     5641 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     5701 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     5761 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     5821 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc
     5881 tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat catggagata
     5941 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     6001 taataaaaaa acctataaat
//
