LOCUS       sequence-319843-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3509
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtaca tgctgctcac cccagaaaga
       61 gtcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttgggtattt ctgtccactg atggactgcg atatagaccg
      241 gggagggatc atgtctaaaa ggtattcacg ctgtcggtct gccaaactgg ggcctttgtg
      301 tcctccgatg ccagtattca agtatgcaat gttgacacct ggcataccaa ggaggccacc
      361 tggcacagac aactggggtg cctgtgcaaa gtttgaaagc atggagcggg cagatgtggg
      421 tatgccacgc tggaggctgc tctggggctg tggagcctgc cgaagcgtat gatgtctcat
      481 gatggtctct tgtttactga cactggacac agctggagcc gtagtggggg agagggctgc
      541 ctgctgctgt aatcgctgtt caatttcctg ttcctgctgt agggctttca caaatgcatt
      601 tttcagccgg ttggtgtgtt cagcttttag agcctttttc tggttggagg tcatacactg
      661 ctcacataga atcttaccat tcttttcttg cttccagtga ggggtgaaat ctgtgcggca
      721 ctgggcacat acaaagggtt caacccgcag aagtgaggca cagcttttgc cttggctgtc
      781 aatgacactc tgtacgactt c
//
