LOCUS       sequence-319846-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3543
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..175)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    183..207
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(667..691)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(744..764)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgtat cgcaataaaa cgtagtattg
       61 ggtcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
      121 tagttcatta ctacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatat
      181 caaccacttt gtacaagaaa gttgggtagg agcccacgac ctggccagac cacgtctcgt
      241 ggggagtgtg gggggccagc tgagtgagca ccacctccac ggcctggaac ttctggttct
      301 tgggagggat agggcacatc ttgtaggtca cctcgtcgcc ctccactggc acgtactccc
      361 cctcgatgtc agacacatgt acgaagatgt cctcggaccc gttctcgggg gtgatgaagc
      421 catggccctg tgagcgtgag aactgcttac agacgccctt gaacacgggg ccagctgagg
      481 cccgggctgt cgctgaatag gtcctggtcc gcttggtggg cagagggctg ggtaggtccc
      541 gaggtgggac accaccccgc tcccagaccc tgctgccctc cctgtggaag gggaaggtgg
      601 gccagactgg ggacttgggg gagtggagcg ggggcacaac tgggggtgac gtcgactctg
      661 aagtcatgcc aacttttttg tacaaacttg ttgatcaatc gatgctagcc tcacgacacc
      721 tgaaatggaa agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga acc
//
