LOCUS       sequence-319851-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the RELA transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0174
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..505)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgtta catacgtccc taagcggtaa
       61 gttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
      121 tagttcatta ctaggagctg atctgactca gcagggctga gaagtccatg tccgcaatgg
      181 aggagaagtc ttcatctcct gaaaggaggc cattggggag ccccggggcc cccagtggag
      241 caggagctgg gtcggggggc ctctgggccc ctgtcactag gcgagttata gcctcagggt
      301 actccatcag catgggctca gttgtgtggg gggccacagg tatgccctgg ttcagcagct
      361 gctgaaactc ggagttgtcg acggatgcca ggtctgtgaa cacagctggg tctgtgctgt
      421 tgccaagcaa ggcccccagg tcttcatcat caaactgcag ctgcagcagg gcctctgaca
      481 gcgttccttc cccagcctgg gtgggcttgg gggcaggtgg ggccacagcc tgaggagggc
      541 ctggggctag gactgggaca ggggctgggg cctgggccag agctgatacc atggctggag
      601 caggggcagg ggctggagcc tggggcagga cttggggagg ggccggggcc aaggc
//
