LOCUS       sequence-319855-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3544
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtccc gtagcctgtc gacccgggtt
       61 atcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttgggtagat atcactggat ttgtacatat ctgaaagcag
      241 tcttgtaatt ggcacattgc cgatggtttt tttgaaaaac acttcttcta tagttgatgg
      301 gctaatagaa cgtaaagctg gcaaaagcaa caggagtttt ccaaagcgac agggttgagt
      361 gggatatctg gtatggatgt agctgttgag cgttagctga gcctcatctt gaagggctgc
      421 aatggcggca gcattccgga aacttctcag ttcagaacca ctatgtgtag gaacggcttt
      481 gaaagtgacg atgcatttta gacaggcaaa ttcagtagca tctaaccgga gttgtctaaa
      541 tcgagccacc acctcttgta aagcctgtat ttcagatatg atcttgttca gcttctggga
      601 atctgtgttg tcaccgttca tgccagatac agccagtaga gtgttagcat caaccggaat
      661 ggcccattgt gctattccta gaacaaacag ttctctccaa gcatcttcca aaagcatcag
      721 ctggtcttgc aaagacagcg tggaga
//
