LOCUS       sequence-319857-        9471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNC4_OE_1
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     463..483
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    535..559
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2309..2333)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2335..2376
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2418..3006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2471..2491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2889..2900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3078..3311
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3389..3510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3426..3445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3480..3499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3550..3685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3705..3724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3706..3724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3734..3751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3734..3750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3743..3765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3892..4347
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3979..3998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4189..4210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4373..4477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4478..4546,4547..5338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4696..4715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5509..6097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5998..6017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6251..6268
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6385..6406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6421..6426,6427..6444,6445..6451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6459..6475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6464..6486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6483..6499
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6483..6499
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6518..6538
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6520..6538
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6566..6792
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6793..6973
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7020..7145
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7638..7871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8056..8100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8275..8775
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8694..8712
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8787..9182
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9242..9359
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      541 ttgtacaaaa aagttggcac catgatcagc tcggtgtgtg tctcctccta ccgcgggcgc
      601 aagtcgggga acaagcctcc gtccaaaaca tgtctgaagg aggagatggc caagggcgag
      661 gcgtcggaga agatcatcat caacgtgggc ggcacgcgac atgagaccta ccgcagcacc
      721 ctgcgcaccc taccgggaac ccgcctcgcc tggctggccg accccgacgg cgggggccgg
      781 cccgagaccg atggcggcgg tgtgggtagc agcggcagca gcggcggcgg gggctgcgag
      841 ttcttcttcg acaggcaccc gggcgtcttc gcctacgtgc tcaactacta ccgcaccggc
      901 aagctgcact gccccgcgga cgtgtgcggg ccgctcttcg aagaggagct caccttctgg
      961 ggcatcgacg agaccgacgt ggaaccctgc tgctggatga cctaccggca gcaccgcgac
     1021 gccgaggagg cgctcgacat cttcgagagc ccggacggag gcggcagcgg cgcggggccc
     1081 agcgacgagg ccggcgacga tgagcgggag ctggccctgc agcgactggg cccccacgag
     1141 ggaggcgcgg gccatggcgc cgggtctggg ggctgccgcg gctggcagcc ccgcatgtgg
     1201 gcgctcttcg aggatcccta ctcctcccgg gccgctaggg tagtggcctt tgcctctctc
     1261 ttcttcatcc tggtctccat caccactttc tgcctggaga cccatgaggc ctttaatatc
     1321 gaccgcaacg tgacagagat cctccgcgta gggaacatca ccagcgtgca cttccggcgg
     1381 gaggtagaga cagagcccat cctgacctac atcgagggcg tatgtgtgct gtggttcaca
     1441 ctggagttcc tggtgcgcat cgtgtgctgc cccgacacgc tggacttcgt caagaacctg
     1501 ctcaacatca tcgactttgt ggccatcctg cccttctacc tggaggtggg gctgagcggc
     1561 ctgtcatcca aggcggcccg cgacgtgctg ggcttcctgc gcgtggtgcg cttcgtgcgc
     1621 atcctgcgta tcttcaagct cacacgccac ttcgtggggc tacgcgtgct gggccacacc
     1681 ctgagggcca gcaccaatga gttcctgctg cttatcatct tcctggccct gggtgtgctc
     1741 atctttgcca ccatgatcta ctacgctgag cgcattgggg ccaggccctc cgaccctcgg
     1801 ggtaatgacc acaccgactt caagaacatc cccattggct tctggtgggc tgtggtcacc
     1861 atgacgacac tgggctacgg agacatgtac cccaagacgt ggtcaggcat gctggtaggg
     1921 gcactgtgtg cactggctgg cgtgctcacc atcgccatgc cggtgcctgt catcgtcaac
     1981 aacttcggca tgtactactc cctggccatg gccaagcaga agctgcccaa gaaacggaag
     2041 aagcacgtgc cacggccggc gcagctggag tcacccatgt actgcaagtc tgaggagact
     2101 tccccccggg acagcacctg cagtgatacc agcccccctg cccgggaaga gggtatgatc
     2161 gagaggaaac gggcaggtga gattaggggt tgggaaggaa aatccctttt cccccagtgg
     2221 cctagggagt ttccaaatgg acctcagacc ttgggatttg gcatgtgttt tgtgtggggc
     2281 ttccctaagc ataaagatgt gcctttattg ccaactttct tgtacaaagt ggttggtaag
     2341 cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
     2401 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2461 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2521 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2581 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2641 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2701 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2761 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2821 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2881 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2941 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3001 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3061 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     3121 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3181 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3241 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3301 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     3361 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3601 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3661 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3721 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4321 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4381 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4441 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4501 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4561 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4621 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4681 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4741 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4801 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4861 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4921 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4981 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5041 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5101 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5161 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5221 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5281 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6181 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6241 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6301 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6361 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6421 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6481 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6541 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6601 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6661 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6721 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     6781 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6961 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7021 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7081 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     7141 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7201 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7261 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7321 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7381 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7441 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7501 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7561 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7621 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7681 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7741 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7801 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7861 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7921 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     7981 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8041 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8101 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8161 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8221 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     8281 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     8341 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     8401 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     8461 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     8521 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     8581 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     8641 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     8701 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     8761 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
     8821 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     8881 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     8941 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     9001 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     9061 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     9121 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     9181 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     9241 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9301 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9361 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt
     9421 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//
