LOCUS       sequence-319863-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3545
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..176)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    184..208
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtg ctgccgtgac agtcagattt
       61 gattcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacgga
      121 ctagttcatt actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata
      181 tcaaccactt tgtacaagaa agttggcaag tccaatgaaa taatgtctgg tatgatgcca
      241 aagatggatg ccgtgtccgt gctgctcctg tttgtgacgg tgtggctatg gctttccctt
      301 aggctgttgg aagaaaccag gctgctgtta ctgccactac tgcttccatt ggtggttggc
      361 agagaagtac tgcctcctgc acttaactga tcaagaaaca tctgtgagga ggtatacggg
      421 aaaaaccgag acgagtgtag gaggtcttga tcatctgaaa ctgctgctgc tgcagcggca
      481 agcaaggagg tgttgtaatg ctgattgtct cctgataaga aaggaaagaa atctaatcct
      541 tgtaagtcgt aaggcatggg tgtcatgtgg aaaggatgcc tatagtcttg gatgagggag
      601 gtattaatgt agcttgtgtc tacagcagga aggcttgggg tgcgggatac tggagatgct
      661 tgatgtggaa gacttaaaat gcctttatta tttagtggtg atgtgggaga tagggaagga
      721 caggtcctct tggcagatgg ctcttcttcc tcttcatcag atgaactgtc tatggttagg
      781 tcaatcactt ctactttc
//
