LOCUS       sequence-319865-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the RELA transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0176
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..501)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 tgtaatccag aggttgattg tcgacttaac gcgttatctg aagactcccg tcctcgcgtc
       61 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggactagt
      121 tcattactag gagctgatct gactcagcag ggctgagaag tccatgtccg caatggagga
      181 gaagtcttca tctcctgaaa ggaggccatt ggggagcccc ggggccccca gtggagcagg
      241 agctgggtcg gggggcctct gggcccctgt cactaggcga gttatagcct cagggtactc
      301 catcagcatg ggctcagttg tgtggggggc cacaggtatg ccctggttca gcagctgctg
      361 aaactcggag ttgtcgacgg atgccaggtc tgtgaacaca gctgggtctg tgctgttgcc
      421 aagcaaggcc cccaggtctt catcatcaaa ctgcagctgc agcagggcct ctgacagcgt
      481 tccttcccca gcctgggtgg gcttgggggc aggtggggcc acagcctgag gagggcctgg
      541 ggctaggact gggacagggg ctggggcctg ggccagagct gataccatgg ctggagcagg
      601 ggcaggggct ggagcctggg gcaggacttg gggaggggcc ggggccaagg ccga
//
