LOCUS       sequence-319868-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3546
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..175)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    183..207
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(439..463)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          492..695
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(515..535)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgtta gtgcagaaaa ctacatcgcg
       61 cgtcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
      121 tagttcatta ctacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatat
      181 caaccacttt gtacaagaaa gttgggtaga ccgcagaacc accaggggcc tcgggcactt
      241 gtggggattc gggagctggc tcttcagggc tcagacagga ctggaacttc tccagctgct
      301 ctgggcttgc cagggtcttc aacagggtgt tctcacgctc tagctgggag ttcttctcca
      361 ccagctctcg gatctgctcc ttcaggatct ccacctcctc tctcacagca tacatcagat
      421 gattcttcac cagatccatg cccacttttt tgtacaaact tgttgatcaa tcgatgctag
      481 cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg
      541 aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct
      601 ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc
      661 ccccaaccta aagacgacgt actccaaaag ctcgagaaa
//
