LOCUS       sequence-319874-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3548
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..176)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    184..208
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtg ccccagagat aggctgatag
       61 aagtcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacgga
      121 ctagttcatt actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata
      181 tcaaccactt tgtacaagaa agttgggtaa catgtttctt ctttcacctg tacagccata
      241 taagacaaat cactctgttc actcttgata tcccagtctg tatctgtttc tgaatcactg
      301 gagtaaatgt caaagagaga ggaagttcta gaccacaatt cctctgtctg agtagttgca
      361 ttacacagtc tttttccaaa cgctttcact ttggcttgaa tacccacact acggtgacgg
      421 ggccttttca aagaaatttg tgtactttta tgcactgggc cttcttcaca gtgagtaggt
      481 actgaagtga cagtattaga tggattatca gcatggggtt gggtttgcac aagttcattt
      541 tcacacgtaa tctgtgaagc tgcagcctgc ttcccagctc gggggcgtgt acaggagact
      601 ggacctgggg cagcctcaga atgcctggct gcctggagct ctcctcgcgt gtccaggcgg
      661 cctgcttggt ctccctcctc tcccctctt
//
