LOCUS       sequence-319877-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the LIN28A transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0087
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..74)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(108..131)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgaga tatcaaactc attactaacc ggttcagtgg
       61 tggtgatggt ggtgaccacc agtttcgggt aaagagcctc ctccggcctt gtcgtcgtcg
      121 tccttgtagt cgcagctctt gggctccacc ctcttgtcca ccttggtgtt gctgggcttg
      181 tggttcacgt tgcagatgta ggtctgggtg cctaaagagc tgctgggcac tgtcaccacg
      241 ctgcttaaag agtataaacc gctggactgc agcacagcgg gaaaggtgtg cacgccgctt
      301 gttaaagctc cgctgttcca gctcacggtc acgggctcgg ggaagtagtc cttcactaaa
      361 cagcccagag cagctgtgcc tccgctggcg ctgctcacgg tcactaaagt accttggccc
      421 cagtagtcga aggggccggt ggtcacggct gtcttgtcac gagcgcagta gtacacggcg
      481 gtgtcctcgg cccttaaaga gttcatctgg aggtataaag tgttcttgct cttgtcacga
      541 gagatggtga aacgaccctt cacgctgtcg gcgtagtcct tgttgctgcc gtcgtaccag
      601 atcacggcca cccattctaa acccttaccg g
//
