LOCUS       sequence-319878-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144[3072 nt]~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtcn atatnagcag
       61 agctggttta gtgaaccgtc agatccgcta gggatcctct agtcagctga cgcgtgctag
      121 cgcggccgcg ttctgccccc agcccctcac agtgcttttc aagccatgct tcctgtgccc
      181 ccagtggggc cctggctggg atatcatcat atactgtaag tttgcgatga gacactacag
      241 tatagatgat gtactagtcc gggcaccccc agctctggag cctgacaagg aggacaggag
      301 agatgctgca agcccaaggg taccgaattc ccatgaaatc tgtcaagaca gattcagaac
      361 aaaatggatc ttctacaatg gccaacgcta ctaaattgtt acacaaggat ggtcaaggtt
      421 tattcgatat tccagagggt atgaatagag gcattaagcc gatggattct ccagctgaaa
      481 atgaggatca aaacggacag ttcaaagaat tgaaacagct aaatgagata gataatgaat
      541 tagacataag gattgaggcg aacgaggcaa agttaaaaga ggaggaaaaa tctgccaagt
      601 caatttctga agaaattatg gaggaagcca ctgaagaaac gactgccgat aatgctgatg
      661 atgccgaaat cgatgaacta ttaccaattg gaatagactt tggcagaact aaaccagttt
      721 cgaaaagtgt tcctgttaaa aaggaatggg cccatgttgt tgatttgaac cataagatcg
      781 aaaatttcga tgagttgatt ccaaaccccg ctagatcgtg gccttttg
//
