LOCUS       sequence-319882-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3513
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..175)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    183..207
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgtag ttggagcttc cgacccttgg
       61 gttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
      121 tagttcatta ctacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatat
      181 caaccacttt gtacaagaaa gttgggtaat ttatggactg gtactctgag tacttcttgt
      241 ggtgcagcag cagaaccaag ccgcccccta gcagcatgag ccctggggca cccagggcca
      301 ctgccatgat gcccaggact agtggggaca agccgtccac tggagggaag cccacaccca
      361 ggagcatcga ccagctgagg tagtgttggt cccaatagcc agggcctgtg gaagccccga
      421 acgtcagatt gaaggcacag aagttattct gggacccaaa gaaggctcgg acaatgggtg
      481 actggggaag agagtatgct aaggcaggat gaagagggga agcttggcag ggcagggctg
      541 attctcggcc ccccggcttc tgggagtaag ccactggtcg ccactgtgca aagcctgatg
      601 ggagggagcc ccacagtagc tggtccaact ggaagacggc cggtgcatat tcatcgtcga
      661 tggagtgctg ctcctgcatt gaggggcagt cagggccctg gcccaatgtg gctacctcca
      721 gcccaaacag ggaacggttt ccccgggg
//
