LOCUS       sequence-319894-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3515
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtgag gtaaatagat tattagtcaa
       61 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttgggtacag gctctcatga tctctgatgc ctggggcctc
      241 agcatcttcc ttggtagcct cttcctcttc atcctcctct tcttcttcct cttggggagg
      301 cccccggcca gagccgggct cagagggggt gctattcttt tccacctcca taggaagggg
      361 cctctcaacc tccaaggtgg ctgcctcctt ctcctctcct tcagggtttt ctgcctcctt
      421 gggacgttta ggagagtcct ccagcaagtc ccctgctctc ctcttcaacg ctcctttctc
      481 gttcttctcc ttggctggct catcaatgac cagcttccct tcctcgtcgc tgctgccctc
      541 tgcattcccc ttcttatcac cgtcaccctc tgcagcttcg ggctctggtt caggctcttc
      601 cacacagctc tttttctggg aggactgata gccggaagcc ttgacagtag ggttgttttc
      661 gatctcccac agcccctcgc tgaacccttt cctcttgttg ggcttgccaa acttctcctt
      721 gga
//
