LOCUS       sequence-319901-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the SMAD7 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0111
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 annnnnnatc tttccnattt tgtaatccag aggttgattg tcgacttaac gcgtccggat
       61 accacttcag ttgcaacttc gtcctttcca caagatatat aaagccaaga aatcgaaata
      121 ctttcaagtt acggactagt tcattactac cggctgttga agatgacctc tagccagcac
      181 gggcagctgc tgatgaactg gcgggtgtag cactggcccc agcccttcac aaagctgatc
      241 tgcacggtaa agcccgtcca cggctgctgc ataaactcgt ggtcattggg ccgctgcagg
      301 ctgtacgcct tctcgtagtc gaaagccttg atggagaaac cggggaacac cttgtgtacc
      361 aacagcgtcc tggagtccgg gttgtccagt gtggcggact tgatgaagat ggggtaactg
      421 ctgcggttgt acacccacac accatccacc tcccgcgtca gctggatgcc gcagccgatt
      481 ttgctccgca ccttctgcac cagctgactc ttgttgtccg aattgagctg tccgaggcaa
      541 aagccattcc cctgaggtag atcatagaag atatccagag agggctcctg gacacagtag
      601 agcctcccca ctctcgtctt ctcctcccag tatgccacca cgcaccagtg tgaccgatcc
      661 ccaggctcca gaagaagttg ggaatctgaa agccccccag gggccagata attcgttccc
      721 cctgtttcag cggaggaagg cacagcatct ggacagtctg cagttggttt gagaaaatcc
      781 atggtggcgg tgctagccnt cacgacacct gaaatggaag aaaaaaactt t
//
