LOCUS       sequence-319903-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3514
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..177)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    185..209
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cttaacgcgt gttgctggtg ggagtagtaa
       61 gtactcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
      121 actagttcat tactacgtag aatcgagacc gaggagaggg ttagggatag gcttaccgat
      181 atcaaccact ttgtacaaga aagttggcaa atcctcatcc acctcctcac cctctgactc
      241 ttcgccgccg ccgctgcccc caccacgtcg ctcataaagc ttatccctct gtcgttcctg
      301 gatttctttc atctcatccg cataccggca aaaggcgcct ccgaacttgc cccaggcttt
      361 gaagggtacg gtgatggcat tgcggtagga cggcttcacc tcgctcactc gcagaaacac
      421 cccgtatttg ttgcagccca catcgaagaa gaagcgcttg gagtccacgg tgatggaggt
      481 gccctccggg agctctccat acaggccgcc ccctgggccc ccggcgccgc ctcccgggcc
      541 gcctgccagc tcgtcgtcct cgcctccgta gtcgtctatg agcttcgcca gcgcgtcgcg
      601 gaactcgatg aggccctgcg caggcagcgc gatggtctgg ccgctctgca agccgcccgg
      661 cccggggccc gcgccgaagc cgccac
//
