LOCUS       sequence-319914-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3525
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtcac cgcggttgcc gcggagtagg
       61 ctcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttgggtaaat gaaggtactc ttggtctgtt ttagatttag
      241 acttagcagt gttttatctt gttgatcttg ttcaaggatc ttaaaatctt ccaaaggaag
      301 actgcttaag gcagttggta acatgtgttc tgatgtacct ttaggtaata cactatttgc
      361 ttctaaattc tttaccaaac acgtggcttt gatccatctt cgagttgctc tgcgttcctt
      421 ttgtaggcaa gttttcattt ttcttcttaa gcttgctatt tctttttcca gtttaatgat
      481 cctctttttt gcctccatag gattcctaaa ggcatagctg tgttcaagta gtacttgctg
      541 actactaatg tttgatttta aattagatgg tccagctgga gaacaactgt tgtttttctt
      601 caaaagattc cttgacttga gtttcataga ctttatatgg gtacaaaaat caaaaatggt
      661 tggaacagca tccattttaa gtcgtcgagt ttgtcctgtt aggtcaaaac aggaggcttc
      721 aaagtgcttt gaacaaagaa aagtgtgttt tcctggcaca aaatttttgc gcctaaccag
      781 gcgaacccat tcttttct
//
