LOCUS       sequence-319922-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the MAX transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0049
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             208..219
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(634..654)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtcaa cgtctacggt cgggcttgag
       61 atcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tagctggcct ccatccggag cttcttcctg ctttggggct cttcaggctc
      181 agactccgag ctggagtccg agcccccatc gaaggcagag atggtgctgc ccttggcgtt
      241 ggtgtagagg ctgttgtctg aggaggggta gttggtctgc agttgggcac ttgacctcgc
      301 cttctccagt gcacggactt gctgctccag aagagcattc tgccgcttga ggtcgtcaat
      361 atcttgctgg tgtgtgtggt ttttccttcg catatactgg atatattctg tggctttgtc
      421 taggatttgg gcccgggatg ccttctctcc ttggagtgat gggactgagt cccgcaaact
      481 gtgaaagctg tctttgatgt ggtccctacg ttttcgttcc agtgcattat gatgagcccg
      541 tttgtcagcg tcgctctcca cctcgatgtc atcgttatcg ctcatggtgg cggtgctagc
      601 ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga
      661 accaagatcc aaactcaaaa
//
