LOCUS       sequence-319931-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3539
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..175)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    183..207
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgtca aggtcccact gggtataaac
       61 attcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
      121 tagttcatta ctacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatat
      181 caaccacttt gtacaagaaa gttgggcagc ccaggagacc cggcggccac agggtcgggc
      241 ttctccaggg ctcccgtccc ttccctttgt ctttcctctt ccttgtttct ggaatctgct
      301 gggtccgctt ctgtcggaca cttcgccacc tccggatcct gggcagccgg atcccacagt
      361 tcggccttct cctccaccca ggagatgagc gccggcttgc tgcctccgac tcctggggga
      421 gaagaacgca aaccccacgc tgcgaggagg ccgcctgccc ggccccgggg cccccaactc
      481 cacacgcagc tcccagaggc gcggcagggc ctgtggaggc gcagggccca ggcaagcagg
      541 tggggctctc accgagcgcg cccaggtggc cgtaggtctc ccgcatcacg tcccggtaca
      601 gggccctctg cgcgggccgc aggcagcccc actcctcccg ggagaagtac acggccacgt
      661 cggcgaagct cacggccccg ggctttctcc actcggatcc cgccccgttt gggtcccggg
      721 gagggagcgg ggccag
//
