LOCUS       sequence-319934-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3526
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtcac ggccgagctg ggaacaccgc
       61 ctcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttggcaaggt attttccaca gtttcggaat tatctgtagg
      241 aagctctgac ttacttgtat agagtttaat atatgtgtcc accattaaat ccaggtcgtg
      301 ttttatatca aaatttatgt taagcaaagc caagttactt gacctttggt ctgtcaaagt
      361 gttcctcaaa tatgctttaa gacgctttcg tccattttca taccgctcat tctcaacctt
      421 catcacagga agaatacaca ggaccttcag caatgcatac acattaggaa aaaacttgat
      481 gtcaggcagg tggagggctt catagatggt ggacggaagc tctatatctt tccccctgtg
      541 tttccatttg attctccaac aatgaagctc agctgacagc gtgtcaggat tgggtaagtc
      601 acttctatac atgtcagcat ggtgttcctc cgacgtattg aatttgagtt gtcccatgac
      661 tgagggtacc agagataagc atttaagagc tttgaggtgc tgttctgaga atatatcttt
      721 aagttcctga ataatgtgct
//
