LOCUS       sequence-319940-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3518
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..174)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(615..639)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcgtacc aatgtccgaa acgttgatgg
       61 atcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggact
      121 agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct taccgatatc
      181 aaccactttg tacaagaaag ttggcaacac gtccaggacg tggttgagat aggagatgta
      241 gcagatggcc aggcgcagga tctcgatctt ggagagcttc ttgtccgggg gcagcgtggg
      301 cagcaatttg cggagctcgg cgaaggccaa gttgaaggct tccacgcgga tgcgctcgcg
      361 ggtggcgtgg gccgagcggt acttggccgt ggcgcgccgg cggcggcgct tctcctcgcg
      421 gctcagctgc tgcgggtgcg ggtagagcgc ggctcggctg ccgcccttgc cgtcgccctc
      481 ggcctcctcc accggctcca ggtccgacac gctgccgagc accttggtgt ccgtgccgcc
      541 cagggactcc ggatccgagt gcgccgagct gggatggtcc gaatctgctg cttggtccgg
      601 actcagcatc atggtgccta cttttttgta caaacttgtt gatcaatcga tgctagcctc
      661 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ct
//
