LOCUS       sequence-319944-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3537
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..186)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    194..218
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcatctttcc aattttgtaa tccagaggtt gattgtcgac ttaacgcgta aaatagctta
       61 tatagcagta ccgtcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
      121 aagttacgga ctagttcatt actacgtaga atcgagaccg aggagagggt tagggatagg
      181 cttaccgata tcaaccactt tgtacaagaa agttgggtat aaggtcgtca ggtccgtgtg
      241 gtggtctttg gccatcggct gtaaatgctg gctgggggcg actgaggagg agcaagatga
      301 ggaggaagat gaggacgatg acctgccttt agtgttgggc agcttatgat cttttttcca
      361 cttcatcctc cggttctgga accagatctt gatctggcgc tccgacagac acagggtgtg
      421 agcgatttca atccgacggc gccttgtcag atacctgtta aaatgaaatt ctttttccag
      481 ttctaggact tgctgccggg tgtaggccgt tcgggaccgc ttgggttccc caccggtgta
      541 gttggggttc accgaattca cgtgcacctt cttcatccag gggtagacca cggccggctg
      601 cttgagtgcc gtcccggagg gcggctgctt ggggtcggac tgactgtagg cccgggcgcc
      661 aggcaggggc gccggcggag gcgccggggg agctgggcaa ggctctcctg gagcggcgta
      721 gtgaccgccg gggccgcctg gctcttgtcc gtgaccccgc gcaggcagcg ccganccngg
      781 cccggggccn ctg
//
