LOCUS       sequence-319947-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3542
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..177)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    185..209
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(588..612)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(664..684)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cttaacgcgt tatcggctag tgcgtacccc
       61 ggcatcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
      121 actagttcat tactacgtag aatcgagacc gaggagaggg ttagggatag gcttaccgat
      181 atcaaccact ttgtacaaga aagttgggca ccataggatt attttgccct cactttccca
      241 ctctttcctt aagtttaaat tctgagggcc acagctccca tatccatcca atatcacttt
      301 ttggaatgat gcttctataa gcttctttct taagaggcct tgagtatcat gagaaaagat
      361 atctggctgg atggctactg tctctgcact cttcacattc cagggctctt tcctttgctc
      421 caaaaaggtg atcaggtccg gcttagagac agcgagaccc agagagacca ggtttccgta
      481 gttctctaac atcacatccc catataaaag cttctgatct gagtccaggt gttcccactc
      541 ctcccgagag aattctatgg ccacatccct gaatgtcaac agtcccatgc caactttttt
      601 gtacaaactt gttgatcaat cgatgctagc ctcacgacac ctgaaatgga agaaaaaaac
      661 tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt
      721 ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc cactc
//
