LOCUS       sequence-320-exp        6420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6420)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD159.62
FEATURES             Location/Qualifiers
     source          1..6420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3867..3889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3989..4008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4086..4104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4172..4276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4277..4345,4346..5137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4495..4514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5308..5896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5797..5816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6050..6067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6184..6205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6220..6225,6226..6243,6244..6250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6258..6274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6263..6285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6282..6298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6282..6298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaaataag cttgggctgc aggtcggcta taataagttc ttgaataaaa taattttccc
       61 gacaaaacat gagtatttct ttcgaaaata aaagtgcagg ctaattagag attattctgt
      121 aattaactgc ataatttgtc acgtgccata gttttacatt ccactacgtc atagttctta
      181 aaatactaat ctcctgaaaa tagaagtagg tgaagaaagt ttaattatca gttctaaaat
      241 gacaattgat ctttggaata tgttctgaaa ctaccgatca ttgaacagat gctatttgaa
      301 tgatatagaa ttgtatattt gcaatttctg aaacgcgttc ttaaaggcac acagattaat
      361 tcaaaagggt ctggccgcaa aaaggtttat ggtggccgat tttgagtttt gtgtgtgatt
      421 gctttttcac aatcagtgtt ttcaggatta tgtgatgaac tagatcttca agtttcgtta
      481 catttcatat gttttcggaa ctcacgaagt acatattggg tattgtgctc aaaaaattca
      541 gcaatcagct tcgctccgct gactttagaa cccaaaaaaa tagtatggcc aaactgactg
      601 tgttacgatc atttcaattt ttcaatacat atttaagatt tctaagagta agaaggtcaa
      661 aaactgttct ggaatacata tatatttttc aggttacaat tagtcaaaaa gtgcactgaa
      721 atatacgttt taatttcacg aataacccaa ttagttcaat gtatttttgg tcaaccaacg
      781 ttaaagtttg gcttccaacc aattatcatt tctgatcaac cacaatgttt tttctttatc
      841 tgcaagttaa ttttttattt ttatccagat gtttggcata tttttcaatt cttcactagc
      901 gcccacttct tgcacttccg gcgccctgaa tctaatgcat ctgttgcaag aattgaaaga
      961 ccaatcaaca cattgttttc ttcacgagat actgaagaaa atgaataaaa acagagaaaa
     1021 agagccatgt gattagtgac aactgttgct aacagatacc atagcttgga cttggtacgt
     1081 gatggcaacg tatgggtcaa caaaaatgat tgcagagggg gtgcaaaaca gtcaagtcga
     1141 gaaaatatga aaaacagaaa acaaagaaca gaaaaatggg tttgagagtc agtataattt
     1201 ataaaagaaa aattgtacat agaaattaac catttttgta gaagaagtta tttttcaagc
     1261 atcgttaaaa attattcaaa gcaccttatt tcatatttaa ttttaaacat ggttaaatga
     1321 acaacacggt gcgcaatcag gaaaacttga aatctgaaac tgttgttgtg atcttcttcg
     1381 caactgttca gatagcacta gtgtaatgtt aagagtgcgc gaatataatg gaatataatg
     1441 gatcacacct cctgccatca ggtaaacgtc tctgttatca catatttcca actattaaat
     1501 ttttaccttt tacagtttta catttttttg aaaaaagtaa ctttttgtct tcaaaatccc
     1561 tgacgaaaat atcaaatatt ttaatcgaga ctgcagagga accgattgat gatttggaaa
     1621 atccagcttt acctgtgtaa gaactgaaaa gtttcataac cctagggtat tcccagttac
     1681 attccccact ggctaacaat agcacccagt ttttcatcac ctttcttcaa atttctcggc
     1741 gatttgttaa aaacaaaatt tgtgtccctt ctctgatatc tctatgtctc taaacacaag
     1801 ttcatcggaa aacgaaggag ggtaggtgtt ggttgggctc ccgaagtgaa aatagaagag
     1861 caagaataga atattagaga gagagtgcag agagggcggg atagctcccg ggattccgtt
     1921 ttcttcttct ttatcttcaa cgatgatgtg tgtgcgtgtt gtatagattc tgttgctccc
     1981 ccacaactcg ctccgaaggc tcaatacaat tcaattgata ttggaggaga gcctaccgga
     2041 gtgggaggat aagaagaaac ataagaagaa gaagaagaag aagcatgctt ctggtttttg
     2101 atgctatgaa aacggcacaa aaagatgatt gaggtccctt ttcaatacct tctctcatct
     2161 ttcaaatccc attgaaacct aaaacttctc accacgcttt accattgttc tccaaaaact
     2221 tatagcaatg tctataactt ttttatctct gaaaagcagt gttccatttt tctttttcct
     2281 attttatttc aattgtttct cacatttcgt ttggattctt tgcttgtcaa ccagcttctt
     2341 cttccacttt taccgtctaa ttttcagggc agggagccat caaacccacg accactagat
     2401 ccatctagag gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
     2461 acataacata gaacattttc aggaggaccc ttggagggta ccggtcgcca ccatgaggtc
     2521 ttccaagaat gttatcaagg agttcatgag gtttaaggtt cgcatggaag gaacggtcaa
     2581 tgggcacgag tttgaaatag aaggcgaagg agaggggagg ccatacgaag gccacaatac
     2641 cgtaaagctt aaggtaacca aggggggacc tttgccattt gcttgggata ttttgtcacc
     2701 acaatttcag tatggaagca aggtatatgt caagcaccct gccgacatac cagactataa
     2761 aaagctgtca tttcctgaag gatttgtaag tttaaacggg gtgttactaa ctaacaaata
     2821 acatttaaat tttcagaaat gggaaagggt catgaacttt gaagacggtg gcgtcgctac
     2881 tgtcacccaa gattccagct tgcaagatgg ctgtttcatc tacaaggtca agttcattgg
     2941 cgtgaacttt ccttccgatg gacctgttat gcaaaagaag acaatgggct gggaagccag
     3001 cactgagcgt ttgtatcctc gtgatggcgt gttgaaagga gagattcata aggctctgaa
     3061 gctgaaagac gggtaagttt aaactatatc ttactaacta actgtagaga tttaaatttt
     3121 cagtggtcat tacctagttg aattcaaaag tatttacatg gcaaagaagc ctgtgcagct
     3181 accagggtac tactatgttg actccaaact ggatataaca agccacaacg aagactatac
     3241 aatcgttgag cagtatgaaa gaaccgaggg acgccaccat ctgttccttt agcggccgcg
     3301 actctagaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     3361 taataggcct actagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa
     3421 ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg
     3481 tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct
     3541 ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt
     3601 tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac
     3661 aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat
     3721 atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa
     3781 aaataagcaa agaaattagg cgaacgatga gaattgtcct cgcttgggcc ctttcgtctc
     3841 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     3901 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     3961 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4021 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     4081 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     5701 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6121 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6181 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6241 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6301 tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat
     6361 tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa caacttggaa
//
