LOCUS       sequence-320028-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-451 under the U6 promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-mir451 EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        345..556
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt
       61 tgagactata agtatccctt ggagaaccac cttgttggaa gaagctctct gctcagcctg
      121 tcacaaccta ctgactgcca gggcacttgg gaatggcaag gaaaccgtta ccattactga
      181 gtttagtaat ggtaatggtt ctcttgctat acccagaaaa cgtgccagga agagaactca
      241 ggaccctgaa gcagactact ggaagggaga ctccactcga gtactaggat ccattaggcg
      301 gccgcgtgga taaccgtatt accgccatgc atgtgcccgt cagtgggcag agcgcacatc
      361 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      421 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      481 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      541 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      601 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      661 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      721 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      781 gcaccttcgc gcctgtctcg c
//
