LOCUS       sequence-320029-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144~451 under the U6 promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-mir144~451 EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..38
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        534..745
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg
       61 ttttgagact ataagtatcc cttggagaac caccttgttg ggttctgccc ccagcccctc
      121 acagtgcttt tcaagccatg cttcctgtgc ccccagtggg gccctggctg ggatatcatc
      181 atatactgta agtttgcgat gagacactac agtatagatg atgtactagt ccgggcaccc
      241 ccagctctgg agcctgacaa ggaggacagg agagatgctg caagcccaag aagctctctg
      301 ctcagcctgt cacaacctac tgactgccag ggcacttggg aatggcaagg aaaccgttac
      361 cattactgag tttagtaatg gtaatggttc tcttgctata cccagaaaac gtgccaggaa
      421 gagaactcag gaccctgaag cagactactg gaagggagac tccactcgag tactaggatc
      481 cattaggcgg ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga
      541 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      601 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      661 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      721 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      781 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      841 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      901 agccccttcg cctcgtgctt gagttgaggc ctgg
//
