LOCUS       sequence-320035-        8511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA,
            Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, 
            Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, 
            DePinho RA, Rimm DL, Chin L
  TITLE     Proinvasion metastasis drivers in early-stage melanoma are 
            oncogenes.
  JOURNAL   Cancer Cell. 2011 Jul 12;20(1):92-103.
  PUBMED    21741599
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell."; date: "2011-07-12"; volume: "20(1)"; pages: "92-103"
COMMENT     Sequence Label: pLenti6/V5-DEST-FASCIN
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(451..475)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(571..595)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(592..795)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(621..641)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        796..1099
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(1378..1422)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1607..1840
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2333..2458
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2505..2685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2686..2912)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2940..2960)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2940..2958)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2979..2995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2979..2995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2992..3014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3003..3019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3027..3033,3034..3051,3052..3057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3072..3093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3210..3227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3381..3969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3461..3480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4140..4931,4932..5000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4763..4782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5001..5105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5131..5586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5268..5289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5480..5499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5713..5735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5727..5744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5728..5744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5754..5773
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5754..5772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5793..5928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5968..6089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5979..5998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6033..6052
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6161..6394
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6481..6879)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6898..6945)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6993..7322)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7007..7142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7307..7327
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7364..7405)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7458..7482
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcttacgggt gacgctgtag atgttgacct gagggtgcat ggcgatgtgc acgctccact
       61 tctcggcggg ggacaccgtc tgcgcgaagc aggacaggcg gtcctcggtg ccgccgaagt
      121 agcgccggtg cgcctcggac tgcagcgacc agcgaccgtc gtcgtgcgcc acgatgagga
      181 aacggcagtc gggaccgggc acctcgcgct cgcaggtcac gttgccgtcc ttgtccgccg
      241 ccaggtagcg gcccaggtgg ctgcgcaggc acacggccgc gctgcccgcc tcgtcagggg
      301 gctgctccag cgtccagatc tgcttcttct tcaggctgct ggcggacgcg ttcaccttga
      361 acccgaacgc ctcggccgtc aggtacttgt tgccgcagtt gatgaggccg aactggatct
      421 gcaccgcctc ggctgtgccg ttggcggtca tgccaacttt tttgtacaaa cttgttgata
      481 tctgcagaat tccaccacac tggactagtg gatcctctag agtcggtgtc ttctatggag
      541 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
      601 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      661 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      721 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      781 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      841 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      901 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      961 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     1021 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     1081 taccgtaagt tatgtaacgc ggaactccca agcttatcga taccgtcgag atccgttcac
     1141 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
     1201 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
     1261 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
     1321 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
     1381 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
     1441 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
     1501 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
     1561 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
     1621 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
     1681 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
     1741 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
     1801 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc
     1861 tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac
     1921 tactttatat ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct
     1981 gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     2041 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     2101 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     2161 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     2221 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     2281 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     2341 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     2401 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     2461 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     2521 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     2581 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     2641 gccagagagc tcccaggctc agatctggtc taaccagaga gacccgttta tgtatcgagc
     2701 taggcactta aatacaatat ctctgcaatg cggcaattca gtggttcgtc caatccatgt
     2761 cagacccgtc tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg
     2821 gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt
     2881 gcaagactac aagagtattg cataagacta cattaagctt gcagctccag cttttgttcc
     2941 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     3001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5101 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     5161 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     5221 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     5281 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     5341 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     5401 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     5461 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     5521 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     5581 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5641 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5701 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     5761 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
     5821 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5881 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5941 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
     6001 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6061 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
     6121 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
     6181 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     6241 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     6301 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     6361 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     6421 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct cgaattgtgc
     6481 ttagccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
     6541 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
     6601 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
     6661 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
     6721 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
     6781 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
     6841 aatgagggtg gattcttctt gagacaaagg cttggccatg gtttagttcc tcaccttgtc
     6901 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat
     6961 ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag
     7021 cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata
     7081 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     7141 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata
     7201 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
     7261 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
     7321 acattccaca gaattaattc caaactcatt actaaccggt acgcgtagaa tcgagaccga
     7381 ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagactc gagcggccgc
     7441 cactgtgctg gatatcaacc actttgtaca agaaagttgg gtagtactcc cagagcgagg
     7501 cggggtccac ggtttccgcc gaggccttca ggacgcctgc gtggtcgccc ttcaggtagc
     7561 gcccgcccac cttgatggcc accttgttat agtcgcagaa ctcgaagaag aagtccacag
     7621 gagtgtcgcc gctgctggtg accgcggagt cactgcccac cgtccagtat ttgcctgtgg
     7681 agtctttgat gttgtaggcg ccatcgttga actccagctg gaagacgtca tagctggagc
     7741 ggttggcgtc cagggtgccc gtgaccttgc ggcagccgat gaagccatgc tccccgcgga
     7801 acacgatgat ggggcggttg atgagcttca tgaggaagag ctctgagtcc cctgctgtct
     7861 ccaccgaggc ggccagctgc ccattcttct tggaggtcac aaacttgcca ttggacgccc
     7921 tcagtgtgat gcgccggtca cgccactcga tgtcaaagta gcagctggca ttcttgctgg
     7981 aggcggtgga ctgcacgccc ccggtggccg tcagcgtcca gtacttgccc gtgtgggtac
     8041 ggaaggcaca ctttttggtg tcgcggtcga tctccagctg gaaggtctcc tggtcggtct
     8101 cctcgtcctg attggcagac aggtccatac cctggcgcgt ggacacgttc ctctcgttgg
     8161 ccgcctgcag cacgacctgg gcgcagctct gctccagagc aaagagctcg tccttgccca
     8221 ccttggtggc cttgcccgcc ttgagcgtgc cgctgggccc cgacggcgcc aggtaacggc
     8281 cctcgcagtc gcggaaggcc accttgccgg agcggaactc cagcgtgtag ccagtggccg
     8341 gctcggggcg cgccaccagg cgcccgtcgt ggcgcaggaa gcggtggtcg gcggtctgca
     8401 cgctgtagcg ctggtcctgg aaggcgaggg tgatgagcga gtcgacgccc cagggcacgt
     8461 cgcggtccac ggcgatctcg tcggccggcc gcgcgctcag gtgcgcgtag c
//
