LOCUS       sequence-320045-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express DGCR8 without tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pNoTag-DGCR8
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc gatccatcga tagtgtggtg gaattctgat cgccgccgcc
      121 atggagacag atgagagccc ctctccgctc ccgtgtgggc ccgcaggaga agcggtgatg
      181 gagagccgag ctcgcccctt ccaagcgctg ccccgtgagc agtctccacc acctcccctg
      241 caaacgtcca gtggtgcaga ggtaatggac gttggctctg gtggtgatgg acagtccgaa
      301 ctccctgctg aggacccctt caacttctac ggagcttctc ttctctccaa aggatccttc
      361 tctaagggcc gcctcctcat agacccgaac tgtagtggcc acagcccgcg caccgcccgg
      421 cacgcacctg cggtccggaa gttctcccct gaccttaagt tgcttaagga tgtaaagatt
      481 agcgtgagct ttaccgagag ctgcaggagt aaggacagga aggtgctgta cacaggagca
      541 gagcgcgacg tgcgggcgga gtgcggtctg ctccttagcc ctgtcagtgg ggacgtgcat
      601 gcttgtccct ttggcgggag tgttggtgac ggggtaggca tagggggtga gagtgctgat
      661 aagaaggatg aggagaatga gctggatcag gaaaagagag tggagtatgc agtgctcgat
      721 gagttagaag attttactga caatttggag ctagatgaag aaggagcagg cgggttcacg
      781 gctaaagcaa tcgttcagag agacagagtg gatgaagagg ccttgaattt cccctacgag
      841 gatgactttg acaacgatgt ggatgctctg ctgg
//
