LOCUS       sequence-320048-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible overexpression of  human SPIC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Fazeli PK, Zhang Y, O'Keefe J, Pesaresi T, Lun M, Lawney B, 
            Steinhauser ML
  TITLE     Prolonged fasting drives a program of metabolic inflammation in 
            human adipose tissue.
  JOURNAL   Mol Metab. 2020 Sep 28;42:101082. doi: 10.1016/j.molmet.2020.101082.
  PUBMED    32992039
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Metab."; date: "2020-09-28"; volume: "42:101082. doi"; pages: " 
            10.1016/j.molmet.2020.101082"
COMMENT     Sequence Label: pCW-SPIC
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        172..486
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     459..483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1255..1765
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1684..1702
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1775..2371
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1775..1794)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2271..2291
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2372..2425
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2426..3043,3044..3049,3050..3085,3086..3088,3089..3124,
                     3125..3127,3128..3163,3164..3172)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3212..3232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3630..3641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3846..4079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4151..4272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4188..4207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4242..4261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4312..4447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4447..4518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4519..4587,4588..5379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4737..4756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5550..6138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6039..6058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6292..6309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6426..6447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6462..6467,6468..6485,6486..6492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6500..6516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6505..6527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6524..6540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6524..6540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6559..6579
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6561..6579
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6607..6833
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6834..7014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7061..7186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7679..7912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8097..8141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      481 atcgcctgga gaattgggcc accatgacgt gtgttgaaca agacaagctg ggtcaagcat
      541 ttgaagatgc ttttgaggtt ctgaggcaac attcaactgg agatcttcag tactcgccag
      601 attacagaaa ttacctggct ttaatcaacc atcgtcctca tgtcaaagga aattccagct
      661 gctatggagt gttgcctaca gaggagcctg tctataattg gagaacggta attaacagtg
      721 ctgcggactt ctattttgaa ggaaatattc atcaatctct gcagaacata actgaaaacc
      781 agctggtaca acccactctt ctccagcaaa aggggggaaa aggcaggaag aagctccgac
      841 tgtttgaata ccttcacgaa tccctgtata atccggagat ggcatcttgt attcagtggg
      901 tagataaaac caaaggcatc tttcagtttg tatcaaaaaa caaagaaaaa cttgccgagc
      961 tttgggggaa aagaaaaggc aacaggaaga ccatgactta ccagaaaatg gccagggcac
     1021 tcagaaatta cggaagaagt ggggaaatta ccaaaatccg gaggaagctg acttaccagt
     1081 tcagtgaggc cattctccaa agactctctc catcctattt cctggggaaa gagatcttct
     1141 attcacagtg tgttcaacct gatcaagaat atctcagttt aaataactgg aatgcaaatt
     1201 ataattatac atatgccaat taccatgagc taaatcacca tgattgcttg tgacggggtt
     1261 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     1321 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     1381 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     1441 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     1501 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     1561 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     1621 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     1681 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     1741 ccgaatcacc gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg
     1801 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     1861 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     1921 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     1981 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     2041 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     2101 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     2161 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     2221 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     2281 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     2341 cacctggtgc atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg
     2401 tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg
     2461 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     2521 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     2581 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     2641 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     2701 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     2761 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     2821 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     2881 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     2941 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     3001 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     3061 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     3121 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     3181 ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3241 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3301 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3361 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3421 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3481 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3541 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3601 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3661 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3721 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc
     3781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3841 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4081 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4141 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4261 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     4321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4441 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4501 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4561 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4621 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4681 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4741 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4801 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4861 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4921 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4981 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5041 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5101 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5161 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5221 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5281 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5341 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5401 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5461 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6241 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6301 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6361 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6421 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6481 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6541 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     6601 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     6661 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     6721 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     6781 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     6841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     7561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     7621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     7681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     7741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     7801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     7861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     7921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8281 cgtttcagac ccacctccca accccgaggg gacaattctc gacctcgaga caaatggcag
     8341 tattcatcca caa
//
