LOCUS       sequence-320051-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..118)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    122..155
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    408..525
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttagctct taaacacacc gccgccgtta cacgccaaca aggtggttct ccaagggata
       61 cttatagtct caaaacacac aattacttta cagttagggt gagtttcctt ttgtgctgtt
      121 tataacttcg tataatgtat gctatacgaa gttatagaaa tccaagccta tcatgtaaaa
      181 tgtagctagt attaaaaaga acagattatc tgtcttttat cgcacattaa gcctctatag
      241 ttactaggaa atattatatg caaattaacc ggggcagggg agtagccgag cttctcccac
      301 aagtccgtgc gagggggccg gcgcgggcct agagatggcg cgtcggatct ctagagcggg
      361 cccggtacta accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
      421 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
      481 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
      541 gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct
      601 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
      661 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
      721 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
      781 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
      841 taattccaat aa
//
