LOCUS       sequence-320063-        6100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 574, Emission: 
            596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mStrawberry-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..6100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        20..323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        324..527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     478..498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2015..2725
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2160..2178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2428..2447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2847..2968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2884..2903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2938..2957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2975..3430)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3112..3133)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3324..3343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3457..3561
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3563..3920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3563..3754
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3586..3606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3771..3906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3955..4749
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3955..4749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4009..4028)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4619..4638
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4937..4956)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4981..5028
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5357..5945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5846..5865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
       61 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      121 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      181 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      241 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      301 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      361 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      421 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      481 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      541 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttcccatgtc
      601 caccaggtcc gtgtcctcgt cctcctaccg caggatgttc ggcggcccgg gcaccgcgag
      661 ccggccgagc tccagccgga gctacgtgac tacgtccacc cgcacctaca gcctgggcag
      721 cgcgctgcgc cccagcacca gccgcagcct ctacgcctcg tccccgggcg gcgtgtatgc
      781 cacgcgctcc tctgccgtgc gcctgcggag cagcgtgccc ggggtgcggc tcctgcagga
      841 ctcggtggac ttctcgctgg ccgacgccat caacaccgag ttcaagaaca cccgcaccaa
      901 cgagaaggtg gagctgcagg agctgaatga ccgcttcgcc aactacatcg acaaggtgcg
      961 cttcctggag cagcagaata agatcctgct ggccgagctc gagcagctca agggccaagg
     1021 caagtcgcgc ctgggggacc tctacgagga ggagatgcgg gagctgcgcc ggcaggtgga
     1081 ccagctaacc aacgacaaag cccgcgtcga ggtggagcgc gacaacctgg ccgaggacat
     1141 catgcgcctc cgggagaaat tgcaggagga gatgcttcag agagaggaag ccgaaaacac
     1201 cctgcaatct ttcagacagg atgttgacaa tgcgtctctg gcacgtcttg accttgaacg
     1261 caaagtggaa tctttgcaag aagagattgc ctttttgaag aaactccacg aagaggaaat
     1321 ccaggagctg caggctcaga ttcaggaaca gcatgtccaa atcgatgtgg atgtttccaa
     1381 gcctgacctc acggctgccc tgcgtgacgt acgtcagcaa tatgaaagtg tggctgccaa
     1441 gaacctgcag gaggcagaag aatggtacaa atccaagttt gctgacctct ctgaggctgc
     1501 caaccggaac aatgacgccc tgcgccaggc aaagcaggag tccactgagt accggagaca
     1561 ggtgcagtcc ctcacctgtg aagtggatgc ccttaaagga accaatgagt ccctggaacg
     1621 ccagatgcgt gaaatggaag agaactttgc cgttgaagct gctaactacc aagacactat
     1681 tggccgcctg caggatgaga ttcagaatat gaaggaggaa atggctcgtc accttcgtga
     1741 ataccaagac ctgctcaatg ttaagatggc ccttgacatt gagattgcca cctacaggaa
     1801 gctgctggaa ggcgaggaga gcaggatttc tctgcctctt ccaaactttt cctccctgaa
     1861 cctgagggaa actaatctgg attcactccc tctggttgat acccactcaa aaaggacact
     1921 tctgattaag acggttgaaa ctagagatgg acaggttatc aacgaaactt ctcagcatca
     1981 cgatgacctt gaaggggatc caccggtggc taccatggtg agcaagggcg aggagaataa
     2041 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
     2101 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2161 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc taacccccaa
     2221 cttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     2281 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     2341 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2401 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2461 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag
     2521 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     2581 gaagcccgtg cagctgcccg gcgcctacat cgtcggcatc aagttggaca tcacctccca
     2641 caacgaggac tacaccatcg tggaactgta cgaacgcgcc gagggccgcc actccaccgg
     2701 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
     2761 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2821 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2941 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3001 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3061 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3121 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3181 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3241 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3301 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3361 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3421 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3541 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3601 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3661 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3721 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3781 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3901 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     4801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4921 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4981 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5041 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5101 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5161 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6001 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     6061 agttattaat agtaatcaat tacggggtca ttagttcata
//
