LOCUS       sequence-320065-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and 
            miR-451 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pLentiCRISPRv1.mir451_KO
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..292)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(102..121)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(272..292)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(470..514)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgctatttc tagctctaaa acttaccatt actaaactca gtcggtgttt cgtcctttcc
       61 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      121 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      181 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
      241 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tctctgggtc
      301 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
      361 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
      421 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
      481 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
      541 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
      601 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
      661 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
      721 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
      781 acagatgctg ttgcgcctca atagccctca gcaaattgtt
//
