LOCUS       sequence-320068-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Drosha-FLAG-mEGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pCK-Drosha(UniProt)-FLAG-mEGFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             459..482
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      483..492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(534..555)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aatcatttat tgcagcgctg tacattgata aggatttgga atatgttcat actttcatga
       61 atgtctgctt ctttccacga ttgaaagagt tcattttgaa tcaggattgg aatgacccca
      121 aatcccagct tcagcagtgt tgcttgacac ttaggacaga aggaaaagag ccagacattc
      181 ctctgtacaa gactctgcag acagtgggcc catcccatgc ccgaacctac actgtggctg
      241 tttatttcaa gggagaaaga ataggctgtg ggaaaggacc aagtattcag caagcggaaa
      301 tgggagcagc aatggatgcg cttgaaaaat ataattttcc ccagatggcc catcagaagc
      361 ggttcatcga acggaagtac agacaagagt taaaagaaat gaggtgggaa agagagcatc
      421 aagagagaga gccagatgag actgaagaca tcaagaaaga ctacaaagac gatgacgata
      481 aagccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      541 agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg
      601 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      661 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      721 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      781 ccatctcctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag
//
