LOCUS       sequence-320081-        8813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Sreekanth V, Zhou Q, Kokkonda P, Bermudez-Cabrera HC, Lim D, Law BK,
            Holmes BR, Chaudhary SK, Pergu R, Leger BS, Walker JA, Gifford DK, 
            Sherwood RI, Choudhary A
  TITLE     Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome 
            Editing Outcomes.
  JOURNAL   ACS Cent Sci. 2020 Dec 23;6(12):2228-2237. doi: 
            10.1021/acscentsci.0c00129. Epub 2020 Nov 18.
  PUBMED    33376784
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.0c00129"; journalName: "ACS Cent Sci"; date: 
            "2020-12-23- 23"; volume: "6"; issue: "12"; pages: "2228-2237"
COMMENT     Sequence Label: pX330 L-FKBP-Cas9
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..286
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          523..750
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      762..771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..836
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             843..863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1596..1916
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2841..5339
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             5340..5387
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5415..5432)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5421..5628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5637..5777
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5637..5766
                     /label=AAV2 ITR
     rep_origin      5852..6307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5939..5958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6149..6170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6324..6343)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6443..6465
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6503..6521)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6589..6693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6694..6762,6763..7554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6912..6931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7725..8313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8214..8233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8375..8623
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8375..8395
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8546..8565
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8642..8717
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      121 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      181 cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt
      241 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      301 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      361 tattttttaa ttattttgtg cagcgatggg ggcgaggggc ggggcggggc gaggcggaga
      421 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      481 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      541 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      601 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
      661 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
      721 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg gactataagg
      781 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
      841 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgga tccgacaaga
      901 agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg
      961 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     1021 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
     1081 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     1141 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     1201 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     1261 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     1321 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     1381 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     1441 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     1501 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     1561 atctgatcgc ccagctgccc ggcggaagtg gcagcggagt gcaggtggaa accatctccc
     1621 caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga
     1681 tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta
     1741 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
     1801 gtcagagagc caaactgact atatctccag attatgccta tggtgccact gggcacccag
     1861 gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaggca
     1921 gtggaagcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     1981 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     2041 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     2101 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     2161 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     2221 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     2281 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     2341 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     2401 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     2461 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     2521 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     2581 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     2641 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     2701 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     2761 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     2821 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     2881 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     2941 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     3001 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     3061 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     3121 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     3181 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     3241 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     3301 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     3361 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     3421 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     3481 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     3541 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     3601 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     3661 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     3721 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     3781 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     3841 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     3901 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     3961 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     4021 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     4081 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     4141 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     4201 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     4261 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     4321 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     4381 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     4441 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     4501 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     4561 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     4621 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     4681 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     4741 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     4801 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     4861 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     4921 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     4981 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     5041 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     5101 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     5161 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     5221 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     5281 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca
     5341 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa gaattcctag
     5401 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5461 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5521 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5581 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgcagga
     5641 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     5701 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     5761 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5821 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     5881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     5941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6061 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6121 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6181 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     6241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     6301 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     6361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6481 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6541 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6781 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6841 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6901 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6961 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7021 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7081 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7141 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7201 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7261 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7321 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7381 gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc actggggcca
     7441 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7501 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7561 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7861 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8341 ttcctggcct tttgctggcc ttttgctcac atgtgagggc ctatttccca tgattccttc
     8401 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     8461 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     8521 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     8581 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtctt cgagaagacc
     8641 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     8701 tggcaccgag tcggtgcttt tttgttttag agctagaaat agcaagttaa aataaggcta
     8761 gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt acc
//
