LOCUS       sequence-320089-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a shRNA targeting mouse Fmr1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Shen M, Wang F, Li M, Sah N, Stockton ME, Tidei JJ, Gao Y, 
            Korabelnikov T, Kannan S, Vevea JD, Chapman ER, Bhattacharyya A, van
            Praag H, Zhao X
  TITLE     Reduced mitochondrial fusion and Huntingtin levels contribute to 
            impaired dendritic maturation and behavioral deficits in Fmr1-mutant
            mice.
  JOURNAL   Nat Neurosci. 2019 Mar;22(3):386-400. doi: 
            10.1038/s41593-019-0338-y. Epub 2019 Feb 11.
  PUBMED    30742117
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-019-0338-y"; journalName: "Nat Neurosci"; date: 
            "2019-03"; volume: "22"; issue: "3"; pages: "386-400"
COMMENT     Sequence Label: 61426-hU6-shmFmr1-EF1a-mCherry
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           223..237
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        348..1526
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          579..1517
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1474..1494
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1533..1542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1539..2249
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1684..1702)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1952..1971
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2274..2862
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2327..2347)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2745..2756)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2865..2881)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2866..2882)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3387..3567
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3593..3610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3599..3823
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3869..4297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3956..3975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4166..4187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4306..4326)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4311..4640
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4491..4626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4553..4572
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4688..4735
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4754..5128
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5258..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5295..5314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5349..5368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5428..5444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5428..5444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5441..5463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5452..5468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5476..5482,5483..5500,5501..5506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5521..5542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5659..5676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5830..6418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5910..5929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6589..7380,7381..7449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7212..7231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7450..7554)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7629..7648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7820..8199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8201..8399
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8354..8374
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8417..8597
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8644..8769
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9262..9495
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9680..9724
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10022..10139
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10189..10239,1..198)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10189..10209
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgc gcaccaagtt gtctcttata tagtgaagcc acagatgtat
      241 ataagagaca acttggtgcg ctttttttgc aaagatggat aaagttttaa acagagagga
      301 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      361 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      421 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      481 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      541 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      601 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      661 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      721 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      781 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      841 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      901 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      961 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     1021 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     1081 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     1141 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atgaaggacg cggcgctcgg
     1201 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1261 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1321 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1381 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1441 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1501 tttttcttcc atttcaggtg tcgtgacgta cggccaccat ggtgagcaag ggcgaggagg
     1561 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1621 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1681 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1741 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1801 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1861 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1921 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1981 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     2041 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2101 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     2161 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     2221 ccggcggcat ggacgagctg tacaagtagg aattcgatat caagcttatc ggtaatcaac
     2281 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2341 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2401 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2461 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2521 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2581 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2641 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2701 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2761 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2821 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     2881 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     2941 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     3001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3061 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     3121 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     3181 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     3241 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     3301 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     3361 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3421 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3481 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3541 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     3601 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3661 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3721 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3781 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3841 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4021 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4201 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4261 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4321 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4381 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4441 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4561 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4621 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4681 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4741 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     4801 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     4861 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     4921 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     4981 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     5041 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     5101 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     5161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5221 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5401 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7561 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     7621 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     7681 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     7741 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     7801 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     7861 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7921 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7981 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     8041 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     8101 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     8161 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     8221 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     8281 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     8341 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     8401 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8461 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8521 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8581 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8641 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8701 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8761 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8821 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8881 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8941 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9001 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9061 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     9121 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9181 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9241 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9301 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9361 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9421 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9481 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9541 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9601 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9661 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9721 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9781 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9841 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9901 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9961 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
    10021 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    10081 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    10141 gggtttatta cagggacagc agagatccag tttggttaat tagctagcga gggcctattt
    10201 cccatgattc cttcatattt gcatatacga tacaaggct
//
